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Combination Chemotherapy and Peripheral Blood Stem Cell Transplant Followed By Aldesleukin and Sargramostim in Treating Patients With Inflammatory Stage IIIB or Metastatic Stage IV Breast Cancer

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. Identifier: NCT00003199
Recruitment Status : Completed
First Posted : January 27, 2003
Results First Posted : May 17, 2017
Last Update Posted : July 12, 2017
National Cancer Institute (NCI)
Information provided by (Responsible Party):
Leona Holmberg, Fred Hutchinson Cancer Center

Brief Summary:
This phase II trial studies how well giving combination chemotherapy and peripheral blood stem cell transplant followed by aldesleukin and sargramostim works in treating patients with inflammatory stage IIIB or metastatic stage IV breast cancer. Drugs used in chemotherapy, such as busulfan, melphalan, and thiotepa, work in different ways to stop the growth of tumor cells, either by killing the cells or by stopping them from dividing. A peripheral stem cell transplant may be able to replace blood-forming cells that were destroyed by chemotherapy. This may allow more chemotherapy to be given so that more tumor cells are killed. Aldesleukin may stimulate the white blood cells to kill breast cancer cells. Giving aldesleukin together with sargramostim may kill more tumor cells

Condition or disease Intervention/treatment Phase
Estrogen Receptor-negative Breast Cancer Estrogen Receptor-positive Breast Cancer Inflammatory Breast Cancer Male Breast Cancer Progesterone Receptor-negative Breast Cancer Progesterone Receptor-positive Breast Cancer Stage IIIB Breast Cancer Stage IV Breast Cancer Drug: tamoxifen citrate Drug: busulfan Drug: thiotepa Drug: melphalan Biological: aldesleukin Biological: sargramostim Procedure: peripheral blood stem cell transplantation Radiation: radiation therapy Phase 2

Detailed Description:


I. To determine the event-free survival and survival of patients treated for inflammatory (Stage IIIb) and responsive stage IV breast cancer with BUMELTT and PBSC support and low dose immunotherapy with IL2 and GM-CSF.


II. To determine the toxicity of a combination of low-dose IL-2 and GM-CSF in patients following HDC with BUMELTT and PBSC support.


PREPARATIVE REGIMEN: Patients receive busulfan orally (PO) once every 6 hours on days -8, -7, and -6; melphalan IV over 30 minutes on days -5 and -4; and thiotepa IV over 2 hours on days -3 and -2.

TRANSPLANTATION: Patients undergo autologous peripheral blood stem cell infusion on day 0.

POST-TRANSPLANT THERAPY: All patients receive tamoxifen citrate* PO once daily beginning prior to aldesleukin (IL-2) and sargramostim (GM-CSF) therapy and continuing for 5 years or until relapse (estrogen receptor [ER]- or progesterone receptor [PR]-positive patients) OR until completion of IL-2/GM-CSF therapy (ER-negative or PR-negative patients). Eligible patients receive IL-2 subcutaneously (SC) daily and GM-CSF SC 3 times weekly for 12 weeks beginning 30-100 days after transplantation. Patients may receive radiotherapy after completion of IL-2/GM-CSF treatment if no prior radiotherapy was given before transplantation.

*Stage IV patients not receiving IL-2/GM-CSF therapy who received tamoxifen citrate as part of adjuvant therapy and subsequently failed, receive oral anastrozole once daily for 5 years or until progression instead of tamoxifen.

[*For postmenopausal patients, the choice and duration of hormonal therapy given in addition to or an alternative to tamoxifen therapy will be at the physician's discretion]

Patients are followed up every 3 months for 2 years, every 6 months for 3 years, and then annually thereafter.

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Study Type : Interventional  (Clinical Trial)
Actual Enrollment : 50 participants
Allocation: N/A
Intervention Model: Single Group Assignment
Masking: None (Open Label)
Primary Purpose: Treatment
Official Title: A Phase II Trial for Patients With Inflammatory (Stage IIIB) and Responsive Metastatic Stage IV Breast Cancer Using Busulfan, Melphalan and Thiotepa Followed by Autologous or Syngeneic PBSC Rescue and 12 Weeks of Post-Engraftment Immunotherapy With Low-Dose IL-2 and GM-CSF
Actual Study Start Date : November 1997
Actual Primary Completion Date : December 2009
Actual Study Completion Date : December 2009

Arm Intervention/treatment
Experimental: Arm I
See Detailed Description.
Drug: tamoxifen citrate
Given orally
Other Names:
  • Nolvadex
  • TAM
  • tamoxifen
  • TMX

Drug: busulfan
Given orally
Other Names:
  • BSF
  • BU
  • Misulfan
  • Mitosan
  • Myeloleukon

Drug: thiotepa
Given IV
Other Names:
  • Oncotiotepa
  • Tespamin
  • TSPA

Drug: melphalan
Given IV
Other Names:
  • Alkeran
  • CB-3025
  • L-PAM
  • L-phenylalanine mustard
  • L-Sarcolysin

Biological: aldesleukin
Given SC
Other Names:
  • IL-2
  • Proleukin
  • recombinant human interleukin-2
  • recombinant interleukin-2

Biological: sargramostim
Given SC
Other Names:
  • GM-CSF
  • Leukine
  • Prokine

Procedure: peripheral blood stem cell transplantation
Undergo autologous peripheral blood stem cell infusion
Other Names:
  • PBPC transplantation
  • PBSC transplantation
  • peripheral blood progenitor cell transplantation
  • transplantation, peripheral blood stem cell

Radiation: radiation therapy
May undergo radiotherapy after completion of IL-2/GM-CSF
Other Names:
  • irradiation
  • radiotherapy
  • therapy, radiation

Primary Outcome Measures :
  1. Event-free Survival [ Time Frame: 11 years ]
    Event-free survival of patients treated for inflammatory (Stage IIIb) and responsive stage IV breast cancer with BUMELTT and PBSC support and low dose immunotherapy with IL2 and GM-CSF.

Secondary Outcome Measures :
  1. Overall Survival [ Time Frame: 11 years ]
    Overall survival of patients treated for inflammatory (Stage IIIb) and responsive stage IV breast cancer with BUMELTT and PBSC support and low dose immunotherapy with IL2 and GM-CSF.

  2. Number of Participants With Toxicity of a Combination of Low-dose IL-2 and GM-CSF [ Time Frame: 16 Weeks ]
    IL-2/GM-CSF toxicity assessed using the NCI Toxicity Criteria. Toxicity was defined as any grade 2, 3, 4 or 5 CNS (except grade 0-3 malaise and fatigue) toxicity; any grade 3, 4, or 5 non-CNS or non-hematological toxicity (except grade 0-3 bilirubin); or any grade 4 or 5 hematological toxicity.

Information from the National Library of Medicine

Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the contacts provided below. For general information, Learn About Clinical Studies.

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Ages Eligible for Study:   19 Years to 65 Years   (Adult, Older Adult)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   No

Inclusion Criteria:

  • Patients with inflammatory (stage IIIb) or responsive stage IV breast cancer with metastasis to soft tissue and/or bone; responsive stage IV disease is defined as patients who achieve a PR (>= 50% reduction in measurable tumor burden) or CR following initial chemotherapy for metastatic disease or patients with locally recurrent disease (chest wall/axillary nodes) who are rendered disease-free following surgery or radiation therapy without receiving chemotherapy; bone disease is categorized as responsive if there is demonstrated sclerosis of prior lesions with no new lesions
  • Patients should have received 4-7 cycles of an Adriamycin and/or taxane-based regimen for stage IIIb or stage IV disease; locally recurrent (chest wall/axillary nodes) patients rendered NED by RT or surgery do not need to receive chemotherapy for stage IV disease prior to Cytoxan/Taxol
  • Patient has received Cytoxan 4 gm/m^2 x 1 and Taxol 250 mg/m^2 x 1 per FHCRC protocol 506.03; Cytoxan/Taxol must be given after all other chemotherapy is completed and before transplant
  • Stem cells were collected after mobilization with Cytoxan/Taxol or after mobilization from an FHCRC approved cytokine protocol; if syngeneic collection, PBSC's were collected by using G-CSF according to FHCRC protocol 753; patient has an adequate number of peripheral blood stem cells stored (>= 2.5 x 10^6 CD34+ cells/kg)
  • The patient must have the capacity to give informed consent; the patient must have signed an approved consent form conforming with federal and institutional guidelines
  • Hepatic function: Bilirubin =< 2 mg%; SGOT or SGPT =< 2.5 x institutional normal
  • Renal function: Creatinine =< 2.0 mg/dl or a creatinine clearance >= 50 mg/min
  • Pre-Study tests have been performed as outlined in the Study Calendar
  • Patients will begin IL-2/GM-CSF therapy if they meet the following criteria post-transplant:
  • Can start therapy 30 to 100 days after transplant
  • Karnofsky performance status > 60
  • ANC > 1,000 cells/mm^3 and platelets > 30,000/cells/mm^3 (transfusion independent) for at least 5 days before starting therapy
  • Total bilirubin =< 2.5 x upper limit of normal
  • SGOT =< 2.5 x upper limit of normal
  • Creatinine =< 2.0 mg/dl

Exclusion Criteria:

  • Patients with a Karnofsky Performance Score less than 70
  • Patients with a left ventricular ejection fraction less than 50 % (LVEF must be performed in patients with symptoms of CHF, abnormal cardiac exam or history of Adriamycin therapy total dose > 400 mg/m^2)
  • Patient is pregnant
  • Patient is seropositive for the human immunodeficiency virus
  • Patients with a history of seizures
  • Patients with hypersensitivity to E.coli preparations
  • Patients with active auto-immune disease
  • Patients with clinically significant pulmonary disease, i.e., diffusion capacity corrected < 60% of predicted; patients with pulmonary problems should be evaluated with appropriate pulmonary studies and/or consult
  • Patients with a history of CNS lesion (brain or carcinoid meningitis)
  • Patients with significant active infection precluding transplant
  • Patients who have had more than one prior chemotherapy regimen for stage IV disease or a prior transplant for any stage disease
  • Patients who have had CD34+ selection of their PBSC products
  • Patients will not receive IL-2/GM-CSF therapy if they:
  • Are > 100 days from transplant
  • Have documented disease progression after transplant
  • Have an active infection
  • Manifested cardiac complications during the initial transplant period, including arrhythmias (that required therapy), congestive heart failure, angina, myocardial infarct, or decreased LVEF to < 45%
  • Currently have pericardial effusions, pleural effusions or ascites
  • Manifested pulmonary toxicity during the initial transplant period and have a diffusion capacity corrected =< 60%
  • Are on steroids
  • Currently have a Grade 3 toxicity from BuMelTT
  • If the patient does not wish to receive the therapy

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its identifier (NCT number): NCT00003199

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United States, Washington
Fred Hutchinson Cancer Research Center/Puget Sound Oncology Consortium
Seattle, Washington, United States, 98109
Sponsors and Collaborators
Fred Hutchinson Cancer Center
National Cancer Institute (NCI)
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Principal Investigator: Leona A Holmberg Fred Hutchinson Cancer Research Center/University of Washington Cancer Consortium
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Responsible Party: Leona Holmberg, Principal Investigator, Fred Hutchinson Cancer Center Identifier: NCT00003199    
Other Study ID Numbers: PSOC 1605
NCI-2010-00728 ( Registry Identifier: CTRP (Clinical Trial Reporting Program) )
First Posted: January 27, 2003    Key Record Dates
Results First Posted: May 17, 2017
Last Update Posted: July 12, 2017
Last Verified: June 2017
Additional relevant MeSH terms:
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Breast Neoplasms
Breast Neoplasms, Male
Inflammatory Breast Neoplasms
Neoplasms by Site
Breast Diseases
Skin Diseases
Molecular Mechanisms of Pharmacological Action
Antineoplastic Agents, Alkylating
Alkylating Agents
Antineoplastic Agents
Myeloablative Agonists
Immunosuppressive Agents
Immunologic Factors
Physiological Effects of Drugs
Analgesics, Non-Narcotic
Sensory System Agents
Peripheral Nervous System Agents
Estrogen Antagonists
Hormone Antagonists
Hormones, Hormone Substitutes, and Hormone Antagonists
Antineoplastic Agents, Hormonal