1.01.02 2020:02:20 22:11:59.145 moyamoya 330998 30 1 1 1 NCT03613701 8157113 Affiliated Hospital to Academy of Military Medical Sciences OTHER Relationship Between Endothelial Progenitor Cells and Revascularization Effect of Moyamoya Disease Relationship Between Endothelial Progenitor Cells and Revascularization Effect of Moyamoya Disease REPCREMMD January 2019 Recruiting No September 1, 2017 Actual September 1, 2019 Anticipated December 1, 2020 Anticipated July 29, 2018 August 2, 2018 August 3, 2018 Actual January 29, 2019 January 31, 2019 Actual Sponsor Affiliated Hospital to Academy of Military Medical Sciences OTHER No No Moyamoya disease is a chronic cerebrovascular disease，The typical pathological manifestations are the stenosis or occlusion of the distal internal carotid artery and/or middle cerebral artery, and the proximal anterior cerebral artery. Meanwhile, the abnormal vascular net, which is the smokey vessel, occurs at the bottom of the brain. Currently the pathogenesis of this disease is unknown. Limited studies have reported the expression of endothelial progenitor cells (EPCs) in moyamoya disease, but the results were inconsistent. Some investigators believe that the number of EPCs in peripheral blood of patients with moyamoya disease is increased, while others believe that the number of EPCs in peripheral blood of moyamoya patients is reduced. Therefore, the investigators need to find a more accurate detection method to confirm the growth of EPC in patients with moyamoya disease. At the same time, whether there is endothelial injury in patients with smoke disease, and the expression of endothelial cells (CEC) in patients with smoke disease, there is no research on this aspect at home and abroad. Objective: Detect the expression of endothelial progenitor cells and endothelial cells from peripheral blood of patients with moyamoya disease, and to assess the relationship between clinical characteristics. Design: A single center study, and planned to enroll 120 patients. The present study was to detect the quantities of EPC from peripheral blood in Moyamoya disease by flow cytometry, and to identify the relationship of endothelial progeIlitor cells and effect of the revascularization on Moyamoya disease. The present study also use cerebral ischemia animal model foe intervention experiment, to explore whether EPC can promote vascular remodeling effect of ischemic cerebrovascular disease, and to provide new thought for the treatment of chronic cerebrovascular disorder. Moyamoya Disease Moyamoya disease Endothelial progenitor cells revascularization Observational Yes 6 Months Case-Control Prospective 120 Anticipated Moyamoya disease patients Moyamoya disease patients/Healthy volunteers Expression of endothelial progenitor cells and endothelial cells in peripheral blood Expression of endothelial progenitor cells and endothelial cells in peripheral 2017.9-2018.8 Inclusion Criteria: Whole-brain vessels angiography or magnetic resonance arteriography (MRA) has the following manifestations: stenosis or occlusion of terminal internal carotid artery or the anterior cerebral artery and/or initiating middle cerebral artery; In the arterial phase, the abnormal smokey vascular net near the occlusive or stenosis lesion can be seen. For patients with stable stroke, there was no acute or subacute cerebral infarction or cerebral hemorrhage, and at least 3 months before the last cerebral infarction or cerebral hemorrhage events. Exclusion Criteria: Exclude atherosclerosis, autoimmune diseases, meningitis, intracranial tumors, multiple neurofibromatosis, Down syndrome, craniocerebral trauma, radiation injury, and other underlying diseases that may cause smoke. Acute or subacute cerebral infarction or cerebral hemorrhage were excluded. Accepts Healthy Volunteers All 18 Years 60 Years Adult Health volunteers' inclusion criteria: Age between 18-60; Male or female; Exclusion criteria: Exclude the volunteers with history of cerebrovascular disease and heart disease. Probability Sample Lian Duan, Chief Contact 0086-10-66947156 keyan307@163.com Lian Duan, Chief The 307th Hospital of Military Chinese People's Liberation Army Study Chair The 307th Hospital of Military Chinese People's Liberation Army Recruiting Beijing Beijing 100071 China Lian Duan, Chief Contact 0086-10-66947156 keyan307@163.com No February 21, 2020 D000009072 Moyamoya Disease D000002340 Carotid Artery Diseases D000002561 Cerebrovascular Disorders D000001927 Brain Diseases D000002493 Central Nervous System Diseases D000009422 Nervous System Diseases D000002539 Cerebral Arterial Diseases D000020765 Intracranial Arterial Diseases D000001157 Arterial Occlusive Diseases D000014652 Vascular Diseases D000002318 Cardiovascular Diseases M10615 Moyamoya Disease Moyamoya Disease high M4176 Carotid Artery Diseases low M4393 Cerebrovascular Disorders low M3786 Brain Diseases low M4325 Central Nervous System Diseases low M4371 Cerebral Arterial Diseases low M21105 Intracranial Arterial Diseases low M3046 Arterial Occlusive Diseases low M15983 Vascular Diseases low T3915 Moyamoya Disease Moyamoya Disease high BC10 Nervous System Diseases BC14 Heart and Blood Diseases All All Conditions Rare Rare Diseases