Phase I/II MAHCT w/ TCell Depleted Graft w/ Simultaneous Infusion Conventional and Regulatory T Cell

Recipient Inclusion Criteria:

• Patients with the following diseases that are histopathologically confirmed are eligible

  • Acute leukemia, primary refractory or beyond CR1
  • Chronic myelogenous leukemia (accelerated, blast or second chronic phase)
  • Myelodysplastic syndromes
  • Non-Hodgkin lymphoma with poor risk features not suitable for autologous HCT
• Age <= 60 yo
• Cardiac ejection fraction >= 45%
• Lung diffusion capacity >= 50%
• Calculated creatinine clearance >= 50 cc/min
• SGPT and SGOT <= 2.5 x ULN, unless elevated secondary to disease. Total bilirubin <= 2 x ULN (patients with Gilbert's syndrome may be included at the discretion of the PI or where hemolysis has been excluded
• Availability of a 6/6 HLA matched sibling defined by Class I (HLA -A and B) serologic typing (or higher resolution) and Class II (HLA-DRB1) molecular typing.
• Karnofsky performance status >=70%
• No prior myeloablative therapy or hematopoietic cell transplantation

Recipient Exclusion Criteria

• Seropositive for any of the following: HIV ab, hepatitis B sAg , hep C ab
• Uncontrolled bacterial, viral or fungal infection defined as currently taking antimicrobial therapy and progression of clinical symptoms.
• Uncontrolled CNS disease involvement
• The recipient is pregnant or a lactating female
• Psychosocial circumstances that preclude the patient being able to go through transplant or participate responsibly in follow up care.

Donor Inclusion Criteria

• Age <= 75 years
• Karnofsky performance status of >=70%
• Medical history and PE confirm good health status as defined by institutional standards
• Seronegative for HIV Ag, HIV 1 and HIV 2 ab, HTLV 1 and HTLV 2 ab hepatitis B sAg or PCR+ or hepatitis C ab or PCR+ , negative for the Syphilis treponemal screen and negative for HIV 1 and hepatitis C by NAT (nucleic acid testing) within 30 days of apheresis collection
• Must be 6/6 matched sibling donor as determined by HLA typing
• Female donors of child-bearing potential must have a negative serum or urine beta-HCG test within three weeks of mobilization
• Capable of undergoing leukapheresis, have adequate venous access, and be willing to undergo insertion of a central catheter should leukapheresis via peripheral vein be inadequate
• The donor or legal guardian greater than 18 years of age, capable of signing an IRB-approved consent form.

Donor Exclusion Criteria

• Evidence of active infection or viral hepatitis
• HIV positive
• Lactating female