Sirtuin Mediated Effects of 36 Hour Fast and 1 Hour Exercise
Hypothesis: during fasting and exercise will there be an activation of sirtuins (Sirt1).
Primarily the investigators will investigate Sirt1 activity in muscle- and fat-biopsies after fasting for 36 hours and after 1 hour of ergometer-cycling at 50 Vo2max.
In addition to this the investigators will investigate the effect of fasting and exercise on the velocity of carbamide synthesis, aminoacid rate of turnover and glucose rate of turnover.
|Study Design:||Allocation: Randomized
Endpoint Classification: Efficacy Study
Intervention Model: Crossover Assignment
Masking: Open Label
Primary Purpose: Basic Science
|Official Title:||Sirtuin Mediated Effects of 36 h Fast and 1 h Exercise|
- Sirt1 activity [ Time Frame: 60 min ] [ Designated as safety issue: No ]
- secondary will we investigate the effect of fasting and exercise on the velocity of carbamide synthesis, aminoacid rate of turnover and glucose rate of turnover. [ Time Frame: 4 h ] [ Designated as safety issue: No ]
|Study Start Date:||September 2009|
|Estimated Study Completion Date:||June 2012|
|Estimated Primary Completion Date:||June 2012 (Final data collection date for primary outcome measure)|
Experimental: fasting and exercise
36 h fast and 1 h ergometer cycling 50% VO2max
Other: fast and exercise
fasting 36 h and exercise 1 h 50% VO2max
1 h ergometer cycling 50% VO2max
exercise 1 h 50% VO2max