|
|
Home
Search
Study Topics
Glossary
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
||||||||||||||||||||||||||||||||||||
| Sponsor: | University of Missouri-Columbia |
|---|---|
| Information provided by: | University of Missouri-Columbia |
| ClinicalTrials.gov Identifier: | NCT00975546 |
Purpose
The investigators propose to treat couples who wish to have a child in which the man is HIV-positive and the woman is HIV-negative. The investigators call these couples HIV-discordant. On the average, an HIV-positive man, who does not participate in high-risk activities, will transmit HIV to a female partner one in every one thousand acts of intercourse without a condom. To reduce transmission of HIV, HIV-discordant couples are counseled to avoid intercourse altogether, or to use condoms during every act of intercourse. In order to have a child, these patients can use donor insemination. If they wish to have a natural child of the infected man, they can use a combination of medication of the man to reduce the amount of virus in his semen, and condom use except at the time of ovulation when the woman produces an egg. This reduces the chance of infecting the woman, but studies have shown that about 4% of women will be infected with HIV using this approach. Alternatively, they can use vitro fertilization (IVF) with intra cytoplasmic sperm injection (ICSI) in which eggs are collected from the woman after hormone-stimulation and are fertilized in the laboratory by injecting a single washed sperm from her husband into each egg. The resulting embryos can be transferred to the wife's uterus and/or frozen for later use. These procedures are believed to minimize the risk of HIV transmission (although the number of cases is low), but IVF-ICSI is very expensive and are not an option for everyone. A simpler method used for over 15 years in Europe is to collect the man's semen, wash the sperm in the laboratory, and test the sperm sample for HIV before placing it in the woman's uterus (intrauterine insemination; IUI). Although the risk of HIV transmission to the woman is presumably not zero with this method, over 4000 inseminations reported have not resulted in infection of any female patients or resulting children.
| Condition | Intervention |
|---|---|
|
Infertility HIV Infections |
Other: Sperm washing and testing for HIV contamination Procedure: Intrauterine Insemination |
| Study Type: | Expanded Access |
| Official Title: | Intrauterine Insemination for HIV-discordant Couples |
This study will enroll couples who wish to have a child in which the man is HIV-seropositive and the woman is HIV-seronegative. The couple will be counseled about their reproductive options, including in vitro fertilization (IVF) donor insemination and adoption. The male patient will be using appropriate therapy to reduce the virus in his semen. Semen will undergo specialized washing to reduce viral contamination of sperm. The sperm are first separated from leukocytes and other seminal constituents by centrifugation over a stepwise gradient. Motile sperm will be separated from the resulting pellet by a swim-up step in which washed sperm are overlaid with fresh medium into which sperm migrate. The final sperm suspension will be stored while testing for HIV is performed by reverse transcription polymerase chain reaction (RT-PCR). Specimens found negative for HIV will then be used for IUI (review: Gilling-Smith et al, 2006; Bujan et al 2007). There is presumably some risk of HIV transmission to the woman and resulting child with this approach; however, over 4000 inseminations reported in Europe over the last 15 years have not resulted in infection of any female patient or resulting child. The woman will be followed for a year after the final IUI to assess seroconversion. If a child is born, he or she will be tested for HIV at 3 months of age.
Eligibility| Ages Eligible for Study: | 18 Years to 38 Years |
| Genders Eligible for Study: | Both |
Inclusion Criteria:
Couple must:
Female must:
Male must:
If the couple does not achieve pregnancy after IUI, they may continue in the study for 5 additional treatment subsequent cycles. The man must continue to receive care for his HIV and the disease must continue to be under control. Before each cycle of insemination, the couple must each sign an attestation statement that he/she is following safe sex practices, and have repeat testing for sexually-transmitted infections.
Contacts and Locations| Contact: Erma Z Drobnis, PhD | 573-817-3124 | drobnise@health.missouri.edu |
| Contact: Becky Sherwood, RN | 573-817-3105 | sherwoodr@health.missouri.edu |
| United States, Missouri | |
| Missouri Center for Reproductive Medicine & Fertility | |
| Columbia, Missouri, United States, 65201 | |
| Contact: Erma Z Drobnis, PhD 573-817-3124 drobnise@health.missouri.edu | |
| Principal Investigator: | Danny J Schust, MD | University of Missouri-Columbia |
More Information
| Responsible Party: | University of Missouri-Columbia ( Danny J. Schust, MD ) |
| Study ID Numbers: | HIV-IUI |
| Study First Received: | September 10, 2009 |
| Last Updated: | January 15, 2010 |
| ClinicalTrials.gov Identifier: | NCT00975546 History of Changes |
| Health Authority: | United States: Institutional Review Board |
|
fertility HIV discordant HIV Infertility in HIV-discordant couples HIV seronegativity |
|
Infertility RNA Virus Infections Sexually Transmitted Diseases, Viral Slow Virus Diseases Immune System Diseases Acquired Immunodeficiency Syndrome Genital Diseases, Male Infection |
Immunologic Deficiency Syndromes Genital Diseases, Female Virus Diseases HIV Infections Sexually Transmitted Diseases Lentivirus Infections Retroviridae Infections |
