Corneal Epithelial Wound Healing Difference Between Human Autoserum and Cord Blood Serum

This study has been completed.
Sponsor:
Information provided by:
National Taiwan University Hospital
ClinicalTrials.gov Identifier:
NCT00681642
First received: May 19, 2008
Last updated: January 6, 2011
Last verified: December 2010
  Purpose

Human serum eye drops have been successfully used in the treatment of severe ocular surface disorders and the enhancement of corneal wound healing. Umbilical cord serum is also proven to be effective in treatment of dry eye and persistent corneal epithelial defects. However, there are limited studies comparing the corneal epithelial wound healing promoting effects between these two blood derived products. The purpose of this study is to test the corneal epithelial wound healing promoting effects between auto serum and human cord blood serum. Primary cultured bovine corneal epithelial cells were used as the model to investigate wound healing, cell proliferation and migration by means of scratch corneal wound healing assay evaluation, MTS assay and Boyden chamber migration assay in response to human serum and umbilical cord serum. The concentrations of EGF, TGF-β1, and fibronectin were also compared between human serum and umbilical cord serum with ELISA kits.


Condition
Corneal Epithelial Wound Healing

Study Type: Observational
Study Design: Observational Model: Case Control
Time Perspective: Prospective
Official Title: Phase 1 Study of Comparison of the Effects on Promoting Corneal Epithelial Wound Healing Between Human Autoserum and Cord Blood Serum—in Vitro Cell Culture Experiment

Further study details as provided by National Taiwan University Hospital:

Primary Outcome Measures:
  • Cell proliferation [ Time Frame: 1 week ] [ Designated as safety issue: No ]

Secondary Outcome Measures:
  • Cell migration [ Time Frame: 1 week ] [ Designated as safety issue: No ]

Enrollment: 7
Study Start Date: March 2007
Study Completion Date: March 2008
Primary Completion Date: March 2008 (Final data collection date for primary outcome measure)
Groups/Cohorts
1
Corneal epithelial tissue with wound cultured in human autoserum
2
Corneal epithelial tissue with wound cultured in umbilical cord serum

  Eligibility

Genders Eligible for Study:   Both
Accepts Healthy Volunteers:   Yes
Sampling Method:   Non-Probability Sample
Study Population

Healthy pregnant women underwent smooth cesarean section during labor Healthy blood donor

Criteria

Inclusion Criteria:

  • Healthy pregnant women
  • Cesarean section for labor
  • Complete placenta with umbilical cord retained
  • Healthy individual

Exclusion Criteria:

  • The retained placenta and umbilical cord were not complete
  • Individual with anemia or other hematologic disorder unsuitable for blood donation
  Contacts and Locations
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Please refer to this study by its ClinicalTrials.gov identifier: NCT00681642

Locations
Taiwan
National Taiwan University Hospital, department of Ophthalmology
Taipei, Taiwan, 10047
Sponsors and Collaborators
National Taiwan University Hospital
Investigators
Principal Investigator: Wei-Li Chen, MD, PhD National Taiwan University Hospital, department of Ophthalmology
  More Information

Publications:

Responsible Party: Wei-Li Chen, MD, PhD, Assistant Professor, National Taiwan University Hospital, department of Ophthalmology
ClinicalTrials.gov Identifier: NCT00681642     History of Changes
Other Study ID Numbers: 200702037R
Study First Received: May 19, 2008
Last Updated: January 6, 2011
Health Authority: Taiwan: Department of Health
Taiwan: Institutional Review Board
Taiwan: National Bureau of Controlled Drugs

Keywords provided by National Taiwan University Hospital:
Corneal epithelial wound
Human autoserum
Cord blood

ClinicalTrials.gov processed this record on September 18, 2014