Dietary Strategies to Promote Muscle Protein Anabolism in the Elderly

This study has been completed.
Sponsor:
Collaborator:
National Nutrition and Food Technology Institute
Information provided by (Responsible Party):
Maastricht University Medical Center
ClinicalTrials.gov Identifier:
NCT00557388
First received: November 13, 2007
Last updated: September 13, 2011
Last verified: September 2011
  Purpose

Aging is associated with the loss of lean muscle mass, termed sarcopenia. Food intake and in particular the ingestion of protein or amino acids has been shown to be a powerful stimulus to promote net muscle protein anabolism. However this anabolic response following a meal-like protein bolus seems to be blunted in the elderly as compared to young adults.

The first aim of this proposal is to investigate the post-prandial muscle protein synthesis rates in young and elderly men in response to a meal-like protein bolus after a period of rest or physical activity (study A). The rest trial (REST) will act as a proof-of-principle study to examine the blunted protein synthetic response in the elderly, and as a control trial in comparison with the exercise trial (EXC) to establish the surplus value of physical activity prior to protein intake on muscle protein synthesis.

The second aim of this proposal is to determine the surplus value of an increased quantity of the ingested protein bolus (study B). Large amounts of protein (40 and 60 g) will be compared to a meal-like amount of protein (20 g) as a means to maximize plasma amino acid availability and/or to stimulate muscle protein anabolism.

The third aim of this proposal is to study the differences in quality of the ingested protein bolus (study C). Instead of significantly increasing the quantity of the protein bolus, we will also apply a more practical approach to augment skeletal muscle protein synthesis rates; modifying the digestibility or amino acid composition of a meal-like protein bolus.


Condition Intervention
Sarcopenia
Dietary Supplement: Protein/Amino Acids
Other: Physical exercise

Study Type: Interventional
Study Design: Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Double Blind (Subject, Outcomes Assessor)
Primary Purpose: Prevention
Official Title: Dietary Strategies to Promote Muscle Protein Anabolism in the Elderly

Resource links provided by NLM:


Further study details as provided by Maastricht University Medical Center:

Primary Outcome Measures:
  • All interventions will affect muscle protein synthesis. With the application of amino acid tracer methodology we are able to determine muscle protein synthesis. [ Time Frame: 6 hours ] [ Designated as safety issue: No ]

Secondary Outcome Measures:
  • Differences in rate of uptake/absorption from the intestine. [ Time Frame: 6 hours ] [ Designated as safety issue: No ]

Enrollment: 120
Study Start Date: December 2007
Study Completion Date: November 2010
Primary Completion Date: November 2010 (Final data collection date for primary outcome measure)
Arms Assigned Interventions
Experimental: 1
Young men 18-30 years, BMI < 27 kg/m2
Dietary Supplement: Protein/Amino Acids
Casein Whey Leucine
Other: Physical exercise
The physical exercise protocol will consist of low intensity cycling and light resistance-type exercise; after 5 minutes of self-paced cycling, subjects will perform 6 sets of 10 repetitions on the horizontal leg press machine (Technogym BV, Rotterdam, The Netherlands) and 6 sets of 10 repetitions on the leg extension machine (Technogym BV, Rotterdam, The Netherlands). The first 2 sets of both resistance exercises will be performed at 40% of the subjects' 1RM. Sets 3-4, and 5-6 will be performed at 55% and 75% of 1RM, respectively, with 2 minutes rest intervals between sets.
Experimental: 2
Young men 18-30 years, BMI < 27 kg/m2
Dietary Supplement: Protein/Amino Acids
Casein Whey Leucine
Experimental: 3
Old men 70-85 years, BMI < 27 kg/m2
Dietary Supplement: Protein/Amino Acids
Casein Whey Leucine
Other: Physical exercise
The physical exercise protocol will consist of low intensity cycling and light resistance-type exercise; after 5 minutes of self-paced cycling, subjects will perform 6 sets of 10 repetitions on the horizontal leg press machine (Technogym BV, Rotterdam, The Netherlands) and 6 sets of 10 repetitions on the leg extension machine (Technogym BV, Rotterdam, The Netherlands). The first 2 sets of both resistance exercises will be performed at 40% of the subjects' 1RM. Sets 3-4, and 5-6 will be performed at 55% and 75% of 1RM, respectively, with 2 minutes rest intervals between sets.
Experimental: 4
Old men 70-85 years, BMI < 27 kg/m2
Dietary Supplement: Protein/Amino Acids
Casein Whey Leucine
Experimental: 5
Old men 70-85 years, BMI < 27 kg/m2
Dietary Supplement: Protein/Amino Acids
Casein Whey Leucine
Experimental: 6
Old men 70-85 years, BMI < 27 kg/m2
Dietary Supplement: Protein/Amino Acids
Casein Whey Leucine
Experimental: 7
Old men 70-85 years, BMI < 27 kg/m2
Dietary Supplement: Protein/Amino Acids
Casein Whey Leucine
Experimental: 8
Old men 70-85 years, BMI < 27 kg/m2
Dietary Supplement: Protein/Amino Acids
Casein Whey Leucine
Experimental: 9
Old men 70-85 years, BMI < 27 kg/m2
Dietary Supplement: Protein/Amino Acids
Casein Whey Leucine
Experimental: 10
Old men 70-85 years, BMI < 27 kg/m2
Dietary Supplement: Protein/Amino Acids
Casein Whey Leucine

  Eligibility

Ages Eligible for Study:   18 Years to 85 Years
Genders Eligible for Study:   Male
Accepts Healthy Volunteers:   Yes
Criteria

Inclusion Criteria:

  • In study A, non-obese male subjects (BMI <27) between the age of 18-30 yrs and 70-85 yrs will be selected.
  • In studies B and C, non-obese male subjects (BMI <27) between the age of 70-85 yrs will be selected.

Exclusion Criteria:

  • Type II diabetes or other known diseases
  • Use of medication
  • Female
  • Other ages or BMI than indicated above
  • Participation in any regular exercise program.
  Contacts and Locations
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the Contacts provided below. For general information, see Learn About Clinical Studies.

Please refer to this study by its ClinicalTrials.gov identifier: NCT00557388

Locations
Netherlands
University of Maastricht
Maastricht, Limburg, Netherlands, 6200 MD
Sponsors and Collaborators
Maastricht University Medical Center
National Nutrition and Food Technology Institute
Investigators
Principal Investigator: Luc van Loon, Phd Maastricht University
  More Information

No publications provided by Maastricht University Medical Center

Additional publications automatically indexed to this study by ClinicalTrials.gov Identifier (NCT Number):
Responsible Party: Maastricht University Medical Center
ClinicalTrials.gov Identifier: NCT00557388     History of Changes
Other Study ID Numbers: MEC 07-3-086, TIFN A-1002
Study First Received: November 13, 2007
Last Updated: September 13, 2011
Health Authority: Netherlands: The Central Committee on Research Involving Human Subjects (CCMO)

Keywords provided by Maastricht University Medical Center:
muscle loss
aging
protein
amino acids
exercise
protein synthesis

Additional relevant MeSH terms:
Sarcopenia
Muscular Atrophy
Neuromuscular Manifestations
Neurologic Manifestations
Nervous System Diseases
Atrophy
Pathological Conditions, Anatomical
Signs and Symptoms

ClinicalTrials.gov processed this record on August 20, 2014