Periodontal Tissue Regeneration Using Autologous Periodontal Ligament Stem Cells (PDLSC)

This study is currently recruiting participants. (see Contacts and Locations)
Verified September 2011 by Fourth Military Medical University
Sponsor:
Information provided by (Responsible Party):
Fa-Ming Chen, Fourth Military Medical University
ClinicalTrials.gov Identifier:
NCT01357785
First received: May 18, 2011
Last updated: September 12, 2011
Last verified: September 2011
  Purpose

The purpose of this clinical trial is to both clarify the efficiency of autologous periodontal ligament (PDL) stem cells to regenerate periodontal tissue in periodontitis patients with deep intraosseous defects (>5 mm) and to confirm the safety of using autologous stem cells in clinical periodontal regenerative medicine. This is a single-centre, randomized, placebo-controlled study. This study has been approved by the ethical committees of School of Stomatology, Fourth Military Medical University. The study will be conducted according to the Declaration of Helsinki.


Condition Intervention Phase
Periodontal Pocket
Procedure: Cell therapy
Phase 1

Study Type: Interventional
Study Design: Allocation: Randomized
Endpoint Classification: Safety/Efficacy Study
Intervention Model: Parallel Assignment
Masking: Open Label
Primary Purpose: Treatment
Official Title: Periodontal Tissue Regeneration Using Autologous Periodontal Ligament Stem Cells: Randomized Controlled Clinical Trial

Further study details as provided by Fourth Military Medical University:

Primary Outcome Measures:
  • Periodontal bone regeneration [ Time Frame: 1 year ] [ Designated as safety issue: Yes ]
    The main outcome measures in the study protocol were: rate of increase in alveolar bone height and millimeter of clinical attachement level (CAL) regained. In addition, we examined whether and to what extent adverse events, for which causal relationships with the use of PDL cell were not ruled out, emerged. We set rate of increase in alveolar bone height as the most statistically important outcome (primary outcome).


Secondary Outcome Measures:
  • Clinical parameters [ Time Frame: 3 months -12 months ] [ Designated as safety issue: No ]
    pocket depth (PD), attachment level (AL), and tooth mobility (TM)


Estimated Enrollment: 35
Study Start Date: April 2011
Estimated Study Completion Date: December 2014
Estimated Primary Completion Date: June 2013 (Final data collection date for primary outcome measure)
Intervention Details:
    Procedure: Cell therapy
    The use of periodontal ligament stem cells for periodontal regeneration
  Hide Detailed Description

Detailed Description:

Periodontitis is an inflammatory disease that causes pathological alterations in the tooth-supporting tissues, potentially leading to tooth loss. National surveys have shown that the majority of adults in the population suffer from moderate periodontitis, with up to 15% of the population being affected by severe generalized periodontitis at some stage in their lives. The significant burden of periodontal disease and its impact on general health and patient quality of life point to the clinical need for effective management of this condition. The ultimate goal of periodontal therapy is predictably regeneration of a functional attachment apparatus destroyed by peridontitis, which involves the formation of a new cementum, periodontal ligament (PDL), and alveolar bone. To date, several therapeutic procedures have been attempted to achieve this ambitious purpose, including root surface conditioning, bone graft placement, guided tissue regeneration (GTR) and the use of enamel matrix-derived proteins or growth factors. However, current regenerative techniques that are used either alone or in combination have limitations in attaining complete and predicable regeneration, especially in advanced periodontal defects, where deep intraosseous defects have been shown to be high-risk sites for progression of periodontitis.

According to histologic evidence, the GTR technique combined with bone graft is the most predictable regenerative procedure in the treatment of such periodontal defects, although basically, current available GTR-based therapies are still crude and of poor clinical predictability. During periodontal regenerative procedures, the remaining healthy periodontal ligament (PDL) plays a key role in the regeneration of new compartments, and the cells of PDL tissues are the only cells that seem to have the capacity to form new attachment apparatus.. The regenerative capacity of the PDL itself is attributed to a few progenitor cells maintaining their proliferation and differentiation potential in the periodontium. Recent advances in stem cell biology and regenerative medicine have presented opportunities for tissue engineering in periodontal therapy. To date, a large number of studies have reported that stem cells, in conjunction with different physical matrices and growth factors, have the capacity to regenerate periodontal tissues in vivo.

It has been demonstrated that PDL-derived progenitors are committed to several developmental lineages, i.e., osteoblastic, fibroblastic and cementoblastic. This property determines the PDL cells as a desirable cell population capable of regenerating a functional periodontal attachment apparatus. It has been shown that PDL cells may prevent ankylosis and root resorption and may possibly also produce a new PDL inserted into the tooth and adjacent bone. Moreover, roots covered with alveolar bone cells induce a cementum-like tissue formation, suggesting that cementoblast and osteoblast precursors commonly originate from the alveolar bone. Thus, PDL-derived cell sources are one of the most promising candidates for cell-based therapies and tissue-engineering-associated periodontal regeneration, and positive pre-clinical results have already been achieved both in vitro and in vivo. Taken together, the results of these investigations demonstrate that PDL cells are capable of preventing epithelial down-growth and root resorption, with the periodontal healing pattern being characterized by connective tissue adaptation involving parallel bundles resting on root dentin. The formation of new bone and cementum varies from a complete absence to coverage of parts of the debrided root surfaces mainly at the borders of the defects, to a thin layer of newly formed cementum with complete new bone formation covering the entire previously denuded root surface. The heterogeneity of results obtained may be explained by the small number of specimens used, the different types of periodontal defects observed and the stability of the carriers utilized to deliver the cells (i.e., hyaluronic acid and blood clots). In addition, one may speculate that PDL cell differentiation is highly sensitive to differences in the microenvironment, resulting in different types of periodontal healing. However, the clinical application of autologous PDL-derived cells are currently lacking.

All over the world, periodontitis remains highly prevalent, can lead loss of the affected teeth, and thus threaten quality of life for middle-aged population as far as oral functions are concerned. Unfortunately in clinic, no current periodontal treatments can, at best, heal the scar in the affected region and ever regenerate lost periodontal tissue or normal structure and functionality. Considering that the "mouth" and "teeth" have various aesthetic and functional roles to play, establishing a brand-new treatment that enables the regeneration of periodontal tissue is very important. It is clear that there is both a clinical need for such treatments and a vast patient resource. Interestingly, there is mounting data that demonstrates the induction of periodontal tissue regeneration by PDL stem cells in animal models. In particular, a number of studies show that topical administration of PDL stem cells enhances periodontal tissue regeneration in models of artificial loss of periodontal tissue in beagles and non-human primates. This suggests that PDL stem cells may be safe and effective in the regeneration of periodontal tissues in periodontitis patients.

In the phase I clinical trial for 20 healthy male volunteers by local administration of autologous PDL stem cells, neither adverse reactions nor rise of any antibody were observed (unpublished data). Importantly, several groups have commenced small-scale pilot/feasibility studies, leading sufficient information to move cell-based therapy into the clinical arena. It is therefore we established this clinical protocol to further test the efficiency and safety of stem cells in the treatment of periodontal deep intraosseous defects.

  Eligibility

Ages Eligible for Study:   18 Years to 65 Years
Genders Eligible for Study:   Both
Accepts Healthy Volunteers:   No
Criteria

Inclusion Criteria:

  1. Those diagnosed as having, from radiography and other results, 2- or 3-walled vertical intrabony defect 3 mm deep from the top of the remaining alveolar bone
  2. Those who have accomplished initial preparation and have been showing good compliance
  3. Those with mobility of the tooth to investigate of Degree 2 or less and with width of attached gingiva for which the existing Guided Tissue Regeneration (GTR) and bone graft implantation treatment are considered appropriate
  4. Those for whom supportive periodontal treatment is applicable (the tooth has the potential to be maintain for at least 3 years), in accordance with usual post-operative procedures following flap operation and GTR treatment
  5. Those whose oral hygiene is well established and who are able to perform appropriate tooth brushing and cleaning following instructions of the investigators and/or sub-investigators after investigational drug administration
  6. Those 18-years-old and <65-years-old Those who has at least one tooth (e.g. wisdom tooth) that is need to be extracted and the patient agrees the tooth extraction.
  7. Those who understand the purposes of the trial and are capable of making an independent decision to comply with trial requirements
  8. Those who are able to visit our hospital in accordance with the trial schedule

Exclusion Criteria:

  1. Those administered a calcium antagonist during the 4 weeks preceding the surgery
  2. Those in need of administration of adrenal cortical steroid (equivalent to>20 mg/day of Predonin) within 4 weeks after the surgery
  3. Those scheduled to undergo a surgical operation in the vicinity of the tooth to investigate within 36 weeks after surgery
  4. Those with coexisting mental or consciousness disorder
  5. Those with coexisting malignant tumour or history of the same
  6. Those with coexisting diabetes (HbA1C >6.5%)
  7. Those in an extremely poor nutritional condition (serum albumin concentration <2 g/dL)
  8. Those with 200 mL of blood drawn during the 4 weeks preceding the surgery
  9. Those administered another investigational drug during the 24 h preceding investigational drug administration
  10. Those with coexisting disorder of the kidney, liver, blood and/or circulatory system (Grade 2 or above)
  11. Those who are either pregnant, possibly pregnant or breast-feeding, or who hope to become pregnant during the period of the trial
  12. Those with a previous history of hypersensitivity to any biological active drugs
  13. Those who smoke more than 10 pieces of cigarettes
  14. Those who are involved in the research team of this trial
  15. Others who the investigators or sub-investigators determined as unsuitable for the trial
  Contacts and Locations
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the Contacts provided below. For general information, see Learn About Clinical Studies.

Please refer to this study by its ClinicalTrials.gov identifier: NCT01357785

Contacts
Contact: Fa-Ming Chen, Ph.D. 86 29 8477 6096 cfmsunhh@fmmu.edu.cn

Locations
China, Shaanxi
School of Stomatology, Fourth Military Medical University Recruiting
Xi'an, Shaanxi, China, 710032
Contact: Yan Jin, Ph.D.    86 29 8477 6141    yanjin@fmmu.edu.cn   
Contact: Fa-Ming Chen, Ph.D.    86 29 8477 6096    cfmsunhh@fmmu.edu.cn   
Principal Investigator: Yan Jin, Ph.D.         
Principal Investigator: Fa-Ming Chen, Ph.D.         
Sub-Investigator: Yong-Jie Zhang, Ph.D.         
Sponsors and Collaborators
Fourth Military Medical University
Investigators
Study Director: Songtao Shi, Ph.D. School of Stomatology, Fourth Military Medical Univeristy; Ostrow School of Dentistry, University of Southern California
  More Information

Publications:

Responsible Party: Fa-Ming Chen, Fourth Military Medical University
ClinicalTrials.gov Identifier: NCT01357785     History of Changes
Other Study ID Numbers: 007
Study First Received: May 18, 2011
Last Updated: September 12, 2011
Health Authority: China: Ministry of Health

Keywords provided by Fourth Military Medical University:
Periodontal ligament stem cells
Periodontal regeneration
Clinical trial
Deep intraosseous defects

Additional relevant MeSH terms:
Periodontal Pocket
Periodontitis
Periodontal Diseases
Mouth Diseases
Stomatognathic Diseases

ClinicalTrials.gov processed this record on August 26, 2014