Role of Cytokines in Hepatitis E Virus Infection During Pregnancy
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Purpose
Hepatitis E virus is a public health problem in several countries of the world where safe drinking water is a problem. HEV is an exclusive cause of epidemic hepatitis in general population. HEV infection occurs most frequently in rainy season. The disease affects mainly young adults in the age of 15-40 years.HEV viral infection is of particular concern in pregnancy. It is a potential disaster for mother and child. HEV infection during pregnancy is fulminant and fatal especially if it occurs in third trimester. The mortality in the second trimester is around 20% and reaches upto 45% in the third trimester.
| Condition |
|---|
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Hepatitis Cytokines Pregnancy |
| Study Type: | Observational |
| Study Design: | Observational Model: Case Control Time Perspective: Prospective |
| Official Title: | Role of Cytokines in Hepatitis E Virus Infection During Pregnancy |
- To correlate the levels of cytokines and its genes polymorphisms with the severity of hepatitis in HEV and non-HEV pregnant women. [ Time Frame: 3 years ] [ Designated as safety issue: No ]
- To correlate the outcome of pregnancy with the levels of maternal cytokines and its genes polymorphisms. [ Time Frame: 3 years ] [ Designated as safety issue: No ]
| Estimated Enrollment: | 300 |
| Study Start Date: | August 2009 |
| Estimated Study Completion Date: | July 2012 |
| Estimated Primary Completion Date: | January 2012 (Final data collection date for primary outcome measure) |
| Groups/Cohorts |
|---|
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Hepatitis-E
Pregnant,Acute Viral Hepatitis, Fulminant Hepatic Failure
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Detailed Description:
The study will include pregnant women with acute viral hepatitis and fulminant hepatic failure (jaundice). The women with FHF will be recruited from the medical wards and antenatal wards as all such patients are routinely admitted in the hospital.
The pregnant women with acute viral hepatitis will be recruited either from antenatal clinic and medical outpatient, or from the medical and antenatal wards because pregnant women with AVH are admitted if they have serum bilirubin levels > 15- 20 mg / dl, persistently high bilirubin levels for more than 2-3 weeks, abnormal prothrombin time, evidence of progression of the disease, need parenteral therapy (because of excessive vomiting).
The enrolled subjects will be evaluated on the basis of a pre-designed and pre-tested proforma with respect to history and clinical examination, obstetrics examination and ultrasonography. Ten ml venous blood sample will be drawn from the patient at the time of enrollment detection of hepatotropic viruses (Various serological markers of hepatitis will be done which includes: IgM anti-HAV, HBsAg, IgM anti-HBc, HBeAg, anti-HCV Ab and IgM anti-HEV would be done using commercially available ELISA kits and Extraction of HEV-RNA from serum will be done) & levels of cytokines (IL-6, TGF-beta, IFN-g and TNF-α). All the subjects will be followed- up till delivery. The promoter region of cytokine gene will be amplified by PCR in appropriate reaction conditions using suitable sets of primers. PCR product will be used for studying the polymorphisms by restriction fragment length polymorphism.
The control group would comprise of age and POG matched healthy asymptomatic pregnant women
Follow up
All participants will be followed up till delivery for obstetrical complications, medical complications and pregnancy outcome.
Eligibility| Ages Eligible for Study: | 18 Years to 40 Years |
| Genders Eligible for Study: | Female |
| Accepts Healthy Volunteers: | Yes |
| Sampling Method: | Non-Probability Sample |
Pregnant women of 18-40 years with jaundice and Healthy pregnant women as controls
Inclusion Criteria:
- Diagnostic criteria of acute viral hepatitis - Patients having acute self-limited disease and a serum aspartate aminotransferase elevation of at least 5 fold or clinical jaundice or both.
- Diagnostic criteria of acute liver failure - When after a typical acute onset, the patient becomes deeply jaundiced and goes into hepatic encephalopathy within 4 weeks of the onset of disease with no past history of chronic liver disease.
- Diagnostic criteria of Hepatitis E infection - The serum sample showing HEV IgM positivity and/or HEV-RNA positivity would be considered as HEV infected cases.
Exclusion Criteria:
1. Patients with co-infection with other hepatitis virus. 2. Patients with any other associated diseases. 3. Patients with history of pre-existing liver disease.
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Contacts and Locations| Contact: Dr. Ashok Kumar, MD | 91-11-23232400 ext 4400 | ash64kr@yahoo.com |
| India | |
| Dr. Ashok Kumar | Recruiting |
| New Delhi, Delhi, India, 110002 | |
| Contact: Dr. Ashok Kumar, MD 91-11-23232400 ext 4400 ash64kr@yahoo.com | |
| Principal Investigator: Dr. Ashok Kumar, MD | |
| Principal Investigator: | Dr. Ashok Kumar, MD | Maulana Azad Medical College, New Delhi-110002 |
More Information
Publications:
| Responsible Party: | Professor Ashok Kumar, Department of Obstetrics and Gynecology, Maulana Azad Medical College |
| ClinicalTrials.gov Identifier: | NCT01062321 History of Changes |
| Other Study ID Numbers: | 5/7/239/07/RHN |
| Study First Received: | February 3, 2010 |
| Last Updated: | July 20, 2011 |
| Health Authority: | India: Institutional Review Board |
Keywords provided by Maulana Azad Medical College:
|
Hepatitis E Virus Cytokines Pregnancy |
Additional relevant MeSH terms:
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Hepatitis Hepatitis A Virus Diseases Hepatitis E Liver Diseases |
Digestive System Diseases Hepatitis, Viral, Human Enterovirus Infections Picornaviridae Infections RNA Virus Infections |
ClinicalTrials.gov processed this record on June 13, 2013