Delta-24-RGD for Recurrent Malignant Gliomas - Full Text View

Sponsor:	M.D. Anderson Cancer Center
Information provided by:	M.D. Anderson Cancer Center
ClinicalTrials.gov Identifier:	NCT00805376

Purpose

The goal of this clinical research study is to find the highest tolerable dose of DELTA-24-RGD-4C that can be injected directly into brain tumors and into the surrounding brain tissue where tumor cells can multiply. A second goal is to study how the new drug DELTA-24-RGD-4C affects brain tumor cells and the body in general.

Condition	Intervention	Phase
Brain Cancer Central Nervous System Diseases	Drug: DELTA-24-RGD-4C Procedure: Tumor Removal	Phase I

Study Type:	Interventional
Study Design:	Treatment, Non-Randomized, Open Label, Single Group Assignment, Safety/Efficacy Study
Official Title:	Phase I Trial of Conditionally Replication-Competent Adenovirus (Delta-24-RGD) for Recurrent Malignant Gliomas

Resource links provided by NLM:

MedlinePlus related topics: Brain Cancer Cancer Neurologic Diseases

U.S. FDA Resources

Further study details as provided by M.D. Anderson Cancer Center:

Primary Outcome Measures:

Maximum Tolerated Dose (MTD) [ Time Frame: Assessed during 14 day waiting period between doses. ] [ Designated as safety issue: Yes ]

Estimated Enrollment:	96
Study Start Date:	December 2008
Estimated Primary Completion Date:	December 2021 (Final data collection date for primary outcome measure)

Arms	Assigned Interventions
Group A: Experimental DELTA-24-RGD-4C	Drug: DELTA-24-RGD-4C Surgical procedure precisely injects DELTA-24-RGD-4C through a catheter (small tube) into brain tumor.
Group B: Experimental DELTA-24-RGD-4C + Surgery	Drug: DELTA-24-RGD-4C Surgical procedure precisely injects DELTA-24-RGD-4C through a catheter (small tube) into brain tumor. Procedure: Tumor Removal Surgical Tumor Resection

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Detailed Description:

The Study Drug:

DELTA-24-RGD-4C is a virus that works by killing brain tumor cells. It is made from a common cold virus, called adenovirus, type 5. This virus normally causes the common cold, and most people around the world have been exposed many times to this common, naturally-occurring virus during their lives. Two (2) changes have been made to the adenovirus to make DELTA-24-RGD-4C. The first change was to make the virus only grow in cancer cells. The second change was made to allow DELTA-24-RGD-4C to enter cancer cells more easily. These changes make DELTA-24-RGD-4C more able to multiply and kill cancer cells and less likely to multiply in normal cells.

Screening Tests:

Before you can start treatment on this study, you will have "screening tests" to help the doctor decide if you are eligible to take part in this study. The following tests and procedures will be performed about 2 weeks before you can receive DELTA-24-RGD-4C:

You will have a physical exam, including measurement of vital signs (blood pressure, heart rate, temperature, and breathing rate) and weight.
You will also be given a neurological (nervous system) exam to check general nervous system function, reflexes, and muscle strength.
You will be asked questions about your past medical and surgical history along with some questions about your recurrent malignant glioma.
You will need to tell the study doctor all of the medications and therapies you take, including vitamins, herbs, and supplements.
You will be asked to provide a blood sample (about 1 teaspoon) , a urine sample and a swab of your nose and throat will be taken to detect the presence of the adenovirus.
Blood (about 5 ½ teaspoons) will be drawn for routine tests, as well as to test for HIV and hepatitis. This routine blood draw will include a pregnancy test for women who are able to have children. To take part in this study, the pregnancy test must be negative.
You will also have a magnetic resonance imaging (MRI) scan taken of your brain. Before the MRI scan, a needle may be placed into your vein (intravenous line or "IV") and a dye called gadolinium will be injected. This dye helps show a better picture of the brain.

If the test results show that you are in poor health or have findings that could make the study unsafe for you, you will not be allowed to participate. You and your partner or family member will be told the test results and you will be given a copy of the results.

Study Groups:

Participants will eventually be divided into 2 groups (Groups A and B).

At first, all participants will only be enrolled into Group A. Participants in Group A will receive the study drug but will not have the recurrent malignant glioma tumor removed by surgery.

Enrollment into Group B will not start until the FDA has reviewed initial results on how the treatment for participants in Group A effected brain tumor cells and the body in general. Once approval for enrollment is given, all eligible participants will be assigned to Group B and will receive the study drug and have their tumors removed by surgery. If you are to receive surgery, you will be asked to sign a separate surgical consent form to help further explain the operation.

If you are found to be eligible to take part in this study, you will be assigned to a dose level of DELTA-24-RGD-4C based on when you joined this study. Up 8 dose levels of DELTA-24-RGD-4C will be tested. Three (3) participants will be enrolled at each dose level. The first group of participants will receive the lowest dose level. Each new group will receive a higher dose than the group before it, if no intolerable side effects were seen. This will continue until the highest tolerable dose of DELTA-24-RGD-4C is found. You will be informed of which dose you will receive.

Study Drug Administration:

DELTA-24-RGD-4C is given by a surgical procedure where a neurosurgeon precisely injects DELTA-24-RGD-4C through a catheter (small tube) that is inserted into your brain tumor. You will stay in the hospital and be watched closely afterward for up to 48 hours, but it may be longer if your doctors feel that you are not ready to go home.

After receiving DELTA-24-RGD-4C, all participants must wear a mask. You must also stay away from pregnant women, babies, children under three years of age, elderly people and large groups of people who may have weaker defenses to disease or those who can spread a disease. You must also stay away from other patients who may have immune system problems, such as people with AIDS, or people who are getting chemotherapy or radiation therapy. The study doctors will decide how long you will need to follow these precautions. You should not donate blood or sperm for at least 6 months after receiving DELTA-24-RGD-4C.

Group A:

Before receiving DELTA-24-RGD-4C, participants in Group A will have a sample (biopsy) of brain tumor tissue collected to confirm that recurrent malignant glioma is present. On the morning of the biopsy, you will have an MRI (with dye). A metal frame will be placed on your head and an MRI of your brain will be done. Before the MRI, a dye will be injected to help give the doctors a better picture of your brain. The head frame allows the surgeon to use MRI to identify the precise location of the tumor within the brain. The frame will stay in place for several hours while the procedure is being performed.

A portion of your head will be shaved for the biopsy. You will have a small incision (cut) in your head (about 2-3 inches). The protective covering of the brain (dura) will be opened and a piece of brain tumor will be removed. If, under a microscope, the biopsy shows that the tumor is still malignant glioma, you will then have an injection of DELTA-24-RGD-4C. The anesthesiologists (doctor who gives the anesthesia) may give you medication to make you sedated (anesthesia). They will place a needle into your vein (intravenous line or "IV") to give you this medicine. The anesthesia is given so that you do not feel any pain or discomfort and do not move during the MRI or the surgery.

A small catheter will be placed into the tumor and the metal frame will help guide the proper placement. Some anti-inflammatory medication (a steroid called dexamethasone) will be given in your vein. Then DELTA-24-RGD-4C will be slowly injected into the tumor over about 10 minutes. After the injection, the incision will be closed in a standard and cosmetically acceptable manner.

Group B:

Before receiving DELTA-24-RGD-4C, participants in Group B will have a biopsy to confirm that the tumor is recurrent glioma. The procedure will be exactly the same as described for those in Group A. However, at the end of the biopsy and study drug injection procedure, the catheter will be clipped off next to your skull and a portion of the tube will remain inside the tumor and in place for the next 2 weeks. The scalp will be closed over the clipped catheter to prevent any possibility of infection. The next day, you will have a CT scan to check the tumor and placement of the catheter.

Group A Follow-up Visits:

You will come back to the study center for check-ups at 4, 7,14 and 28 days, then 2, 3, and 4 months after the injections, and then every 2 months for the rest of your life. At each visit, the following tests and procedures will be performed:

You will be asked to give information about any new medications and therapies, including vitamins, herbs, and supplements that you may be taking.
You will be asked to describe any changes in your health or how you feel.
You will have a neurological exam.
Your weight will be measured on Day 28 and at 2, 3 and 4 months.
Your vital signs will be measured.
You will be asked to provide a blood sample (about 1 teaspoon), a urine sample and a swab of your nose and throat will be taken.
You will have blood tests (about 4 teaspoons) to evaluate your clinical status on Days 4, 7, 28, and at 2, 3 and 4 months.
Blood (about 2 teaspoons) will be collected to look for the presence of anti-AD5 and virus.
Women who are able to have children must have a blood (about 1 teaspoon) pregnancy test except for Day 7.
You will have an MRI at each visit except for Day 4.
Every 2 months after the 4 month visit, you will have an evaluation to see how you have responded to treatment.

Group B Follow-up Visits:

You will be seen in the study center for check-ups on Days 4, 7, and 13 after the injection but before your craniotomy. At each visit the following tests and procedures will be performed:

You will be asked to give information about any new medications and therapies, including vitamins, herbs, and supplements that you may be taking.
You will be asked to describe any changes in your health or how you feel.
You will have a neurological exam.
Your vital signs will be measured.
You will be asked to provide a blood sample (about 1 teaspoon), a urine sample and a swab of your nose and throat will be taken.
You will have a blood test (about 4 teaspoons) to evaluate your clinical status.
Blood (about 2 teaspoons) will be collected to look for the presence of anti-AD5 and virus.
Women who are able to have children must have a blood (about 1 teaspoon) pregnancy test except for Day on Day 13.
You will have an MRI on Days 7 and 13 only.

If you are feeling well 14 days after the first injection of study drug, you will be brought back to the operating room for a second procedure to remove the tumor. You will undergo a craniotomy, in which the skull over the tumor is removed to get to the brain and tumor. The neurosurgeon will try to remove the tumor as completely as possible with the catheter tip from the first procedure in place. After the tumor has been removed, you will receive another dose of DELTA-24-RGD-4C that will be injected into the brain tissue that surrounded the tumor to try to kill any remaining tumor cells. The skull will then be replaced and the skin closed. Within 6 hours of the craniotomy on Day 14, you will have a neurological exam and your vital signs will be measured.

Group B Follow-up Visits after Craniotomy:

After the surgery, you will be seen in the hospital or come back to the center on Days 15, 18, 21, and 28, at 2, 3 and 4 months, and then every 2 months for the rest of your life. At each visit, the following tests and procedures will be performed:

You will be asked to give information about any new medications and therapies, including vitamins, herbs, and supplements that you may be taking.
You will be asked to describe any changes in your health or how you feel.
You will have a neurological exam.
Your weight will be measured on Day 28 and at 2, 3 and 4 months only.
Your vital signs will be measured.
You will be asked to provide a blood sample (about 1 teaspoon), a urine sample and a swab of your nose and throat will be taken.
You will have a blood test (about 4 teaspoons) to evaluate your clinical status.
Blood (about 2 teaspoons) will be collected to look for the presence of anti-AD5 and virus.
Women who are able to have children must have a blood (about 1 teaspoon) pregnancy test at all visits except Day 15 and 18.
You will have an MRI.

This is an investigational study. At this time, DELTA-24-RGD-4C is only being used in research. Up to 96 patients will take part in this study (up to 48 in Group A and 48 in Group B). All will be enrolled at M. D. Anderson.

Eligibility

Ages Eligible for Study:	18 Years and older
Genders Eligible for Study:	Both
Accepts Healthy Volunteers:	No

Criteria

Inclusion Criteria:

Patients with histologically proven recurrent malignant primary glioma will be eligible. Glioma type will be restricted to: GBM, gliosarcoma (GS), anaplastic gliomas [anaplastic astrocytoma (AA), anaplastic oligodendroglioma (AO), anaplastic infiltrating glioma (AIG), mixed anaplastic glioma (MAG), anaplastic ependymoma]
Patients must show unequivocal evidence for tumor recurrence or progression by MRI scan within 15 days prior to Day 0/Baseline procedure after failing prior surgical resection, biopsy, chemotherapy or radiation
For patients entered in Group A (see Treatment Plan) tumors must be accessible for stereotactic injection. Tumors must be between 1.0 - 5.0 cm in diameter
For patients entered in Group B (see Treatment Plan) tumors must be surgically resectable, and surgical resection must be indicated at the time of baseline evaluation. Tumors must be >1.0 cm in diameter.
Patients will consent to have a biopsy taken at the time of the stereotactic injection to confirm the presence of malignant glioma (based on frozen section) before injection of Delta-24-RGD-4C
For each patient there must be a consensus between the physician investigators in this study that injection will not delivery Delta-24-RGD-4C into the ventricular system. Patients must have a stable steroid regimen for at least 1 week prior to Delta-24-RGD-4C administration
Patients may or may not have had prior chemotherapy
Patients must be willing and able to give informed consent
Age > /= 18 years
Patients must have a Karnofsky performance status >/= 70
Patients must have recovered from the toxic effects of prior therapy (i.e., CTC grade 1 or less). For example, they must be at least two weeks after vincristine, 6 weeks after nitrosoureas, and 3 weeks after procarbazine or temozolomide administration
Patients must have adequate bone marrow function (absolute granulocyte count > 1,500 and platelet count of > 100,000), adequate liver function (SGPT and alkaline phosphatase < 2 times institutional normals and bilirubin <1.5 mg%), and adequate renal function (BUN or creatinine <1.5 times institutional normal) prior to starting therapy
This study was designed to include women and minorities, but was not designed to measure differences of intervention effects. Males and females will be recruited with no preference to gender
No exclusion to this study will be based on race. Minorities will actively be recruited to participate. The malignant glioma patient population treated at MDACC over the past year is as follows: American Indian or Alaskan Native - 0, Asian or Pacific Islander - <2%, Black, not of Hispanic Origin - 3%, Hispanic - 6%, White, not of Hispanic Origin - 88%, Other or Unknown - 2%, Total - 100%

Exclusion Criteria:

Any radiotherapy within 4 weeks prior to date of Delta-24-RGD administration.
Active uncontrolled infection or severe intercurrent medical conditions. All patients must be afebrile at baseline (i.e., < 38.0 degrees Celsius [C])
Evidence of bleeding diathesis or use of anticoagulant medication or any medication that may increase the risk of bleeding that cannot be stopped prior to surgery. If the medication can be discontinued , based on the clinical judgment of the surgeon, prior to Delta-24-RGD-4C injection then patient may be eligible.
History or current diagnosis of any medical or psychological condition that in the Investigator's opinion, might interfere with the subject's ability to participate or inability to obtain informed consent because of psychiatric or complicating medical problems
Female who is pregnant and/or nursing. Because of the potential risk of a recombinant virus containing a gene involved in cellular growth regulation and differentiation which could potentially affect a developing fetus or growing infant, females who are pregnant, at risk of pregnancy, or breast feeding a baby during the study period are excluded
Tumor position that, in the Investigator's opinion, would pose the risk of penetration of the cerebral ventricular system during injection with study drug. If, during the Delta-24-RGD-4C injection procedure, penetration of the ventricular system is suspected or confirmed, Delta-24-RGD-4C administration will be aborted
Immunocompromised subjects, subjects with autoimmune conditions, active hepatitis (A, B, C or D [Delta]) or HIV seropositivity
Patients with Li-Fraumini Syndrome or with a known germ line deficit in the retinoblastoma gene or its related pathways
Multiple intracranial malignant glioma lesions
Tumor involvement which would require ventricular, brainstem or posterior fossa injection or access through a ventricle in order to deliver the virus
Tumor involving the subependyma or suspected cerebrospinal fluid (CSF) dissemination
Documented extracranial metastasis
Biologic/immunotherapy (e.g., IL-2, IL-12, interferon) within 4 weeks of Delta-24-RGD-4C administration
Concurrent chemotherapy, radiation or biological therapy
Any contraindication for undergoing MRI such as: individuals with pacemakers, epicardial pacer wires, infusion pumps, surgical and/or aneurysm clips, shrapnel, metal prosthesis, implants with potential magnetic properties, metallic bodies in the eyes, etc.
White blood cell (WBC) < 2.5 x 103/mm3, absolute neutrophil count (ANC) < 1.5 x 103/mm3, platelet < 100,000/mm3, hemoglobin (Hgb) < 10.0 gm/dL, prothrombin time/international normalized ratio (PT/INR) or partial thromboplastin time (PTT) > 1.8 x control
Grade 4 hematological toxicity
Serum creatinine > 1.5 mg/dL
Liver transaminases (aspartate aminotransferase [AST] and/or alanine aminotransferase [ALT]) or total bilirubin > 2x the upper limits of normal
Vaccinations of any kind within 30 days prior to Delta-24-RGD-4C administration
History or current diagnosis of other cancer except curative cervical cancer in situ, basal or squamous cell carcinoma of the skin
History of encephalitis, multiple sclerosis, other CNS infection or primary CNS disease that would interfere with subject evaluation
Patients with history of prior gene transfer therapy or prior therapy with cytolytic virus of any type, especially Delta-24-RGD-4C
Males or females who refuse to use a double-barrier form of birth control during the study and for up to 6 months after injection with Delta-24-RGD-4C

Contacts and Locations

Please refer to this study by its ClinicalTrials.gov identifier: NCT00805376

Contacts

Contact: Frederick F. Lang, MD, BS

713-563-2883

Locations

United States, Texas
UT MD Anderson Cancer Center	Recruiting
Houston, Texas, United States, 77030
Principal Investigator: Frederick F. Lang, MD, BS

Sponsors and Collaborators

M.D. Anderson Cancer Center

Investigators

Principal Investigator:

Frederick F. Lang, MD, BS

UT MD Anderson Cancer Center

More Information

Additional Information:

UT MD Anderson Cancer Center website This link exits the ClinicalTrials.gov site

No publications provided

Responsible Party:	UT MD Anderson Cancer Center ( Frederick F. Lang, MD/Assistant Professor )
Study ID Numbers:	ID01-310
Study First Received:	December 8, 2008
Last Updated:	October 2, 2009
ClinicalTrials.gov Identifier:	NCT00805376 History of Changes
Health Authority:	United States: Food and Drug Administration

Keywords provided by M.D. Anderson Cancer Center:

Brain
Brain Cancer
Central Nervous System Diseases
CNS

Conditionally Replication-Competent Adenovirus
Delta-24-RGD
Recurrent Malignant Gliomas
malignant brain tumor

Additional relevant MeSH terms:

Neoplasms by Histologic Type
Neoplasms, Nerve Tissue
Nervous System Diseases
Central Nervous System Diseases
Central Nervous System Neoplasms
Brain Diseases
Brain Neoplasms
Neuroectodermal Tumors

Neoplasms
Neoplasms by Site
Neoplasms, Germ Cell and Embryonal
Glioma
Neoplasms, Neuroepithelial
Nervous System Neoplasms
Neoplasms, Glandular and Epithelial

ClinicalTrials.gov processed this record on November 27, 2009