• The primary objective is to determine the change of FEV1 expressed as percent of predicted after two doses of albuterol (5 mg each) and ipratropium (0.5 mg each) when compared to FEV1 at Hour 2 after the start of the infusion of MN-221 or placebo. [ Time Frame: Hour 2 ] [ Designated as safety issue: No ]
  

• To evaluate the safety, tolerability, and pharmacokinetic profile of MN-221 when administered after two doses of albuterol (5 mg each) and ipratropium (0.5 mg each) in subjects with acute exacerbation of asthma. [ Time Frame: Continuous ] [ Designated as safety issue: No ]
  
• FEV1 % of predicted at time points other than Hour 2 [ Time Frame: Hours 1, 3, 4, 5, and 24 ] [ Designated as safety issue: No ]
  
• FEV1 (L) [ Time Frame: Hours 1, 3, 4, 5, and 24 ] [ Designated as safety issue: No ]
  
• PEFR (L/sec) [ Time Frame: Hours 1, 3, 4, 5, and 24 ] [ Designated as safety issue: No ]
  
• PEFR, expressed as percent (%) of predicted [ Time Frame: Hours 1, 3, 4, 5, and 24 ] [ Designated as safety issue: No ]
  
• Dyspnea index scale [ Time Frame: Hours 1, 2, 3, 4, 5, 24, and Day 8 ] [ Designated as safety issue: No ]
  
• Number of albuterol treatments to achieve FEV1 ≥ 50% [ Time Frame: No specific time points ] [ Designated as safety issue: No ]
  
• Number of albuterol treatments to achieve FEV1 ≥ 70% [ Time Frame: No specific time points ] [ Designated as safety issue: No ]
  
• Time to achieve FEV1 ≥ 50% [ Time Frame: No specific time points ] [ Designated as safety issue: No ]
  
• Time to achieve FEV1 ≥ 70%; [ Time Frame: No specific time points ] [ Designated as safety issue: No ]
  
• Hospital admission rate [ Time Frame: No specific time points ] [ Designated as safety issue: No ]
  
• Hospital length of stay (in hours) [ Time Frame: No specific time points ] [ Designated as safety issue: No ]
  
• ICU admission rate [ Time Frame: No specific time points ] [ Designated as safety issue: No ]
  

This is a randomized, modified single-blind, placebo-controlled dose escalation, multi-center Emergency Department (ED) study. Each subject will receive MN-221 or placebo administered through a continuous intravenous infusion in addition to the standardized care treatment for an acute exacerbation of asthma. The study is a modified single-blind design where the subject and the Investigator will be blinded.

Upon presentation to the ED for assessment and treatment for an acute exacerbation of asthma the subject should receive standardized care consistent with the National Asthma Education and Prevention Program (NAEPP) guidelines.

Once the subject has received the standardized initial treatment regimen and has been assessed for response to that treatment (signs and symptoms of acute asthma exacerbation), an informed consent to participate in the study will be obtained, study entry criteria will be reviewed, a 12-lead ECG will be performed, a dyspnea index scale assessment will be conducted, and spirometry will be performed. If the subject's FEV1 is ≤ 55% of predicted and the subject meets all other study entry criteria the subject will be randomized to receive either MN-221 or placebo. Throughout the screening process the subject will continue to receive the appropriate medical care consistent with the NAEPP guidelines for the treatment of acute exacerbations of asthma.

There will be up to three dose groups with generally twelve subjects in each group. Subjects enrolled in the study will receive an intravenous infusion of MN-221 study drug or placebo. Generally six subjects will be randomized to receive MN-221 and generally six subjects will be randomized to receive placebo in each dose group.

The initial dose group will be randomized to receive:

• 16 μg/min of MN-221 for 15 minutes (total dose of 240 μg) or placebo.

Subsequent dose groups will receive the following proposed doses:

• 30 μg/min for 15 minutes (total dose of 450 μg) or placebo, and
• 16 μg/min for 15 minutes followed by 8 μg/min for 105 minutes (total dose of 1,080 μg) or placebo.

During the study treatment period, the subject will continue to receive the following standard treatment and assessment until the subject's FEV1 reaches ≥ 70% of predicted:

• Assessment of subject's signs and symptoms;
• Complete a dyspnea index scale;
• Supplemental oxygen to maintain oxygen saturation as measured by pulse oximetry of ≥ 90%;
• Albuterol (2.5 mg) via nebulizer given hourly; NOTE: Albuterol (2.5 mg) via nebulizer may be given up to every 20 minutes if deemed to be indicated by the Investigator.
• Ipratropium (0.5 mg) via nebulizer may be given every hour if deemed to be indicated by the Investigator.
• Spirometry completed within 10 minutes of nebulizer treatments; followed by,
• Reassessment of signs and symptoms. If the subject does not improve to FEV1 ≥ 70% of predicted during the study treatment period, the subject may continue to receive further treatment including hospital admission at the discretion of the Investigator. The study will be approximately 6.5 hours in length (Hour -1.5 to Hour 5) while the subject remains in the ED. Safety, efficacy and PK parameters will be monitored throughout the treatment period. An initial 24-hour post-randomization follow-up visit will be completed to evaluate the subject's health status as well as for safety and PK parameters. A second follow-up contact will be completed by telephone seven days post-randomization for safety purposes and to evaluate the subject's health status.

A risk/benefit evaluation will be performed by the study's Safety Review Committee at each dose level. The occurrence of clinical signs, symptoms, laboratory abnormalities, ECG abnormalities suggesting toxicity, or results of efficacy analyses (FEV1, dyspnea index scale), may result in a decision to modify the proposed planned dose escalations, to repeat a dose level, or to not evaluate any additional dose(s) of MN-221.