Effect of Partial Sleep Deprivation on Immunological System in Peripheral Blood

This study is not yet open for participant recruitment. (see Contacts and Locations)
Verified August 2013 by Hadassah Medical Organization
Sponsor:
Information provided by (Responsible Party):
ayman abu rmaileh, Hadassah Medical Organization
ClinicalTrials.gov Identifier:
NCT01930279
First received: August 25, 2013
Last updated: August 27, 2013
Last verified: August 2013

August 25, 2013
August 27, 2013
September 2013
September 2014   (final data collection date for primary outcome measure)
  • surface markers of T cell as assessed by FACS [ Time Frame: 6 months ] [ Designated as safety issue: Yes ]
  • serum cytokine levels [ Time Frame: 6 months ] [ Designated as safety issue: Yes ]
  • immune parameters to be assessed by western including STAT proteins [ Time Frame: 6 months ] [ Designated as safety issue: Yes ]
Same as current
Complete list of historical versions of study NCT01930279 on ClinicalTrials.gov Archive Site
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Effect of Partial Sleep Deprivation on Immunological System in Peripheral Blood
Effect of Sleep Deprivation on Expression of T Cell Markers in Peripheral Blood of Medical Residents

Sleep deprivation was found to affect many organs including the immune system and predisposing for various health consequences including diabetes hypertension infections and increase in neoplastic diseases.

Subjects will be evaluated for immune parameters in peripheral blood test following a regular nigh sleep and compared with a test performed following a night shift in which they slept less than 3 hours.

Each participant will serve as its own control.

Overall Study Design and Plan

  1. Subjects will be bled on the day of the experiment between 8and 9 AM.
  2. Blood tubes will be tested for T cell markers, serum cytokines levels, and other immune parameters by western blots.
  3. The first tube will be tested on the day of experiment.
  4. The second tube will kept in the lab and will be tested 24 hours later.
  5. The third tube will be kept in the pocket of the subject and be tested 24 hours later.
  6. The fourth tube will be kept in the pocket of another subject and will be tested 24 hours later.
  7. The subject will be tested again following 24 hours.
  8. Each subject will be tested twice following a regular 6-8 hours of sleep, and following an on call duty in which he slept less than 3 hours.
Observational
Observational Model: Case Control
Time Perspective: Prospective
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Probability Sample

Medical residnets

Sleep Deprivation
Other: Blood withdrawal
Blood withdrawal
surface markers on T cells as assessed by FACS
Intervention: Other: Blood withdrawal
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*   Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.
 
Not yet recruiting
50
September 2014
September 2014   (final data collection date for primary outcome measure)

Inclusion Criteria:

Male and female ages 18-40 signed confirmed consent no evidence of chronic disease or chronic medications

Exclusion Criteria:

Any type of acute infection or other acute illness

Both
18 Years to 40 Years
Yes
Contact: Ayman Abu rmeileh, MD 0584400010 ext 00972 aymansar@gmail.com
Contact: Tawfik Khoury, MD 0509870611 ext 00972 taufick@hadassah.org.il
Israel
 
NCT01930279
LDB001
Not Provided
ayman abu rmaileh, Hadassah Medical Organization
Hadassah Medical Organization
Not Provided
Not Provided
Hadassah Medical Organization
August 2013

ICMJE     Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP