Investigation of Stem Cells in Human Lipoaspirate

This study is currently recruiting participants. (see Contacts and Locations)
Verified May 2014 by University of Nevada, Las Vegas
Sponsor:
Information provided by (Responsible Party):
William A. Zamboni, M.D., University of Nevada, Las Vegas
ClinicalTrials.gov Identifier:
NCT01677520
First received: August 28, 2012
Last updated: May 1, 2014
Last verified: May 2014

August 28, 2012
May 1, 2014
March 2011
August 2018   (final data collection date for primary outcome measure)
Percent of Viable ADSCs [ Time Frame: Day of Surgery ] [ Designated as safety issue: No ]
Percent of viable ADSCs will be measured on fresh samples and samples refrigerated for different times.
Percent of Viable ADSCs [ Time Frame: Day of Surgery ] [ Designated as safety issue: No ]
Percent of viable ADSCs will be measured on fresh samples and smaples refrigerated for different times.
Complete list of historical versions of study NCT01677520 on ClinicalTrials.gov Archive Site
Percent of Apoptotic ADSCs [ Time Frame: Day of Surgery ] [ Designated as safety issue: No ]
Percent of apoptotic ADSCs will be measured on fresh samples and on refrigerated samples at 1, 2, 3, and 4 days refrigerated storage.
Same as current
Percent of Necrotic ADSCs [ Time Frame: Day of Surgery ] [ Designated as safety issue: No ]
Percent of necrotic ADSCs will be measured on fresh samples and on refrigerated samples at 1, 2, 3, and 4 days of refrigerated storage.
Same as current
 
Investigation of Stem Cells in Human Lipoaspirate
Investigation of Adipose-Derived Stem Cells in Human Lipoaspirate

The overall purpose of this study is to enhance identification and isolation of ADSCs and adipocytes, to find the optimum culturing conditions to increase growth and volume of grafts, and to study the effects of cryopreservation on ADSCs and adipocytes. Fat injections are used in aesthetic surgery but tend to disassemble over time. If the investigators can enhance growth and volume of fat in culture, the investigators could use fat grafts in reconstruction of cancer and traumatic injury defects with the patients own cultured fat cells and grafts.

Not Provided
Observational
Observational Model: Case-Only
Time Perspective: Cross-Sectional
Not Provided
Retention:   Samples Without DNA
Description:

Cells from lipoaspirate maximum of 20 days

Non-Probability Sample

Primary Care Clinic

Breast Cancer
Not Provided
18-30 yrs, 31-50 yrs, 51-70 yrs
No intervention
Not Provided

*   Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.
 
Recruiting
240
August 2018
August 2018   (final data collection date for primary outcome measure)

Inclusion Criteria:

  • All adults (18-70 yrs) who have scheduled surgical lipectomy of the hips, abdomen, or thighs are prospective subjects for this study.

Exclusion Criteria:

  • Pregnant women will be excluded to protect the unborn child.
Both
18 Years to 70 Years
No
Contact: William A Zamboni, M.D. 702-671-2278 wzamboni@medicine.nevada.edu
Contact: Linda L Stephenson, BS,MT(ASCP) 702-895-3315 lstephenson@medicine.nevada.edu
United States
 
NCT01677520
12.08.004
No
William A. Zamboni, M.D., University of Nevada, Las Vegas
University of Nevada, Las Vegas
Not Provided
Principal Investigator: William A Zamboni, M.D. UNSOM
Study Director: Wei Z Wang, M.D. wwang@medicine.nevada.edu
University of Nevada, Las Vegas
May 2014

ICMJE     Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP