Collection of Cerebrospinal Fluid and Tumor Tissue in Subjects With Metastatic Melanoma and Controls

This study has been withdrawn prior to enrollment.
(Trial experienced funding issues, was never opened to accrual.)
Sponsor:
Information provided by:
University of Pittsburgh
ClinicalTrials.gov Identifier:
NCT01312506
First received: March 8, 2011
Last updated: December 5, 2013
Last verified: December 2013

March 8, 2011
December 5, 2013
July 2012
July 2012   (final data collection date for primary outcome measure)
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Complete list of historical versions of study NCT01312506 on ClinicalTrials.gov Archive Site
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Collection of Cerebrospinal Fluid and Tumor Tissue in Subjects With Metastatic Melanoma and Controls
Procurement of Central Nervous System and Extracranial Tumor Tissues, Cerebrospinal Fluid, and Serum/Plasma From Patients With Metastatic Melanoma to the CNS and Respective Controls for Gene Expression and Proteomic Profiling

This study has two ultimate goals:

  1. Understand how the original melanoma from the skin spreads to the CNS. In order to study this we need to collect (and compare) the original skin melanoma, the CNS melanoma, as well as any other melanoma that has not metastasized to the CNS.
  2. Develop a diagnostic blood test that will early identify subjects at high risk for developing CNS metastasis or patients who may already have CNS metastasis but the MRI or the CAT scan cannot yet detect it. Thus, the investigators plan to collect CSF from subjects like you who have melanoma CNS metastases in order to confirm the presence of this biomarker.
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Observational
Observational Model: Case Control
Time Perspective: Prospective
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Non-Probability Sample

Primary care clinic

  • Metastatic Melanoma
  • CNS Metastases
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  • Subjects undergoing a craniotomy
    This group will include those subjects who have consented to have either a craniotomy or a laminectomy to resect an intramedullary tumor.
  • Metasteses, no craniotomy
    These subjects have metastases but have either chosen not to undergo removal of the cancer or their neurosurgeon does not recommend surgical approach or they have been diagnosed with metastatic melanoma but do not have CNS metastases.
  • Healthy Volunteers
    Subjects who do not have known melanoma or metastases.
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*   Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.
 
Withdrawn
0
July 2012
July 2012   (final data collection date for primary outcome measure)

Inclusion Criteria:

  1. Regarding patients with distant metastatic melanoma and established melanoma CNS metastases who are scheduled for craniotomy:

    • Histologically confirmed, primary cutaneous melanoma, metastatic to the CNS (brain OR spinal cord OR carcinomatous meningitis) based on:

      • Pathologic confirmation (i.e. prior craniotomy) OR
      • Radiography (brain MRI or CT scan with intravenous contrast)
    • Patients must have no contraindications for and be able to undergo lumbar puncture for CSF collection
    • Hemoglobin level of 8g/dL or higher. This can be drawn on the day of consent, or will be acceptable if documented from a previous visit within the prior 30 days
    • Subjects must have provided written, informed consent (ICF) prior to any study procedures
    • Subjects must provide a FFPE tissue block containing a biopsy from the primary site
  2. Regarding patients with distant metastatic melanoma and established melanoma CNS metastases who are not having a craniotomy and before they undergo external beam irradiation or stereotactic radiosurgery (patient's and/or neurosurgeon's preference):

    • Histologically confirmed melanoma (unknown primary, mucosal, ocular are allowed), metastatic to the CNS (brain OR spinal cord OR carcinomatous meningitis) based on

      • Pathologic confirmation (i.e. prior craniotomy) and/or
      • Radiography (brain MRI or CT scan with intravenous contrast)
    • Patients must have no contraindications for and be able to undergo lumbar puncture for CSF collection
    • Hemoglobin level of 8g/dL or higher. This can be drawn on the day of consent, or will be acceptable if documented from a previous visit within the prior 30 days
    • Subjects must have provided written ICF prior to any study procedures
  3. Regarding patients who have distant metastatic melanoma without radiographically detectable melanoma CNS metastases but are willing to undergo lumbar puncture:

    • Histologically confirmed melanoma (unknown primary, mucosal, ocular are allowed),
    • Patients must have no contraindications to lumbar puncture
    • Hemoglobin level of 8g/dL or higher. This can be drawn on the day of consent, or will be acceptable, if documented from a previous visit within the prior 30 days
    • Subjects must have provided written ICF prior to any study procedures
  4. Regarding healthy volunteers

    • Patients must have no contraindications to lumbar puncture (see Table 1)
    • Patient must answer NO to all questions listed in Appendix B
    • Hemoglobin level of 8g/dL or higher. This can be drawn on the day of consent or will be acceptable if documented from a previous visit within the prior 30 days
    • Subjects must have provided written, ICF prior to any study procedures

Exclusion Criteria:

  • For study subjects, patients with extradural lesions, i.e. those that originate from the bone (calvarium or vertebrae), will not be considered.
  • Any significant psychiatric disease, medical intervention, or other conditions, which in the opinion of the Investigators, could impair proper discussion of the informed consent or compromise participation to the clinical trial.
  • Active systemic treatment for metastatic melanoma within 2 days from the collection of specimens (brain tumor tissue, peripheral blood, CSF). Corticosteroids, other immunosuppressive anti-inflammatory and anti-epileptic medications are allowed.
  • Patients who have undergone whole brain irradiation therapy within the last 30 days. Therefore neither CNS lesions nor CSF are considered suitable for collection.
  • Patients with growing CNS lesions at an area that has previously undergone radiosurgery within 3 months prior to enrollment in this study. Therefore CNS tumors from previously irradiated areas using radiosurgery are not considered suitable for collection although CSF is allowed for collection.
  • Brain abscess.
  • Patients with new focal findings on neurological examination.
  • Infected skin over the lumbar puncture needle entry site.
  • Other conditions that at the opinion of the investigator are contraindicated.
Both
18 Years and older
Yes
Contact information is only displayed when the study is recruiting subjects
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NCT01312506
10-067
Yes
Stergios Moschos, MD, University of Pittsburgh
University of Pittsburgh
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Principal Investigator: Stergios Moschos, MD University of Pittsburgh
University of Pittsburgh
December 2013

ICMJE     Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP