Spermatogonial Stem Cells in Azoospermic Patients: a Comparison Between Obstructive and Non-obstructive Azoospermia

The recruitment status of this study is unknown because the information has not been verified recently.
Verified August 2010 by Hillel Yaffe Medical Center.
Recruitment status was  Active, not recruiting
Sponsor:
Information provided by:
Hillel Yaffe Medical Center
ClinicalTrials.gov Identifier:
NCT01178463
First received: August 9, 2010
Last updated: NA
Last verified: August 2010
History: No changes posted

August 9, 2010
August 9, 2010
January 2010
May 2010   (final data collection date for primary outcome measure)
SSCs were identified in both obstructive and nonobstructive azoospermia patients. [ Time Frame: One year ] [ Designated as safety issue: No ]
Same as current
No Changes Posted
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Spermatogonial Stem Cells in Azoospermic Patients: a Comparison Between Obstructive and Non-obstructive Azoospermia
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The researchers hypothesized that nonobstructive azoospermia might be associated with a reduction OF SSCs in seminiferous tubules as compared with obstructive azoospermia. Testicular sperm specimens that had been previously extracted from azoospermic patients will be paraffin embedded and stained with anti GPR-125. After antigen retrieval, the sections will be incubated with rabbit anti GPR125 followed by Cy3-conjugated anti rabbit secondary antibodies. Those found to be SSCs will be counted using light microscopy, and compared between patients with obstructive (n=11) and non-obstructive azoospermia (n=9).

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Observational
Observational Model: Cohort
Time Perspective: Retrospective
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Probability Sample

Infertile male patients treated at the Hillel Yaffe Medical Center IVF Unit

Male Infertility
Other: Biospecimen staining
The sections will be incubated with rabbit anti GPR125 followed by Cy3-conjugated anti rabbit secondary antibodies.
  • I. Obstructive Azoospermia
    Intervention: Other: Biospecimen staining
  • II. Non-Obstructive Azoospermia Patients
    Intervention: Other: Biospecimen staining
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*   Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.
 
Active, not recruiting
25
October 2010
May 2010   (final data collection date for primary outcome measure)

Inclusion Criteria:

  • Azoospermic patients

Exclusion Criteria:

  • Patients with sperm cells
Male
18 Years and older
No
Contact information is only displayed when the study is recruiting subjects
Israel
 
NCT01178463
HYMC-0065-09
Yes
Dr. Adrian Ellenbogen, Hillel Yaffe Medical Center
Hillel Yaffe Medical Center
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Hillel Yaffe Medical Center
August 2010

ICMJE     Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP