Use of Existing Fibroblast Cells to Convert to Induced Pluripotent Stem Cells

This study is ongoing, but not recruiting participants.
Sponsor:
Information provided by (Responsible Party):
Hadassah Medical Organization
ClinicalTrials.gov Identifier:
NCT00801372
First received: December 2, 2008
Last updated: October 16, 2013
Last verified: October 2013

December 2, 2008
October 16, 2013
November 2008
December 2014   (final data collection date for primary outcome measure)
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Complete list of historical versions of study NCT00801372 on ClinicalTrials.gov Archive Site
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Use of Existing Fibroblast Cells to Convert to Induced Pluripotent Stem Cells
Use of Existing Fibroblast Cells to Convert to Induced Pluripotent Stem Cells

Induced pluripotent stem cells potentially may be useful in the future as an unlimited source of cells for transplantation.

The major goal of the project is to develop human iPS cells from existing lines of fibroblasts that were originally donated as clinical grade feeders for the development of clinical grade hESCs. The clinical grade feeders were developed from aborted fetuses, foreskin and umbilical cord.

The potential to reprogram somatic cells into an embryonic state raises multiple basic research questions related both to the process of reprogramming and the properties of iPS cells. We will use various approaches to study the molecular mechanisms and processes that occur during reprogramming. We will use various experimental systems to characterize and study the properties of the iPS cells, their biology, developmental potential, capability to give rise to functional differentiated progeny etc.

The clinical grade donated feeders used in this project were developed under GMP conditions using animal-free reagents. For potential future clinical applications, the iPS cells will be developed under GMP conditions, using reagents and methods that will be acceptable for clinical applications.

Observational
Observational Model: Case Control
Time Perspective: Prospective
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Non-Probability Sample

pregnant, healthy females older than 18 who signed an informed consent for the termination of the pregnancy.

pregnant healthy couples older than 18 who signed an informed consent for a c-section.

healthy male newborns, undergoing surgical circumcision who's parents older than 18 signed an informed consent.

  • Pregnant, Healthy Females
  • Healthy Male Newborns
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Pre-existing Fibroblast MCB
Pre-existing fibroblast donors for hESC derivation project
Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007 Nov 30;131(5):861-72.

*   Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.
 
Active, not recruiting
1
December 2014
December 2014   (final data collection date for primary outcome measure)

Inclusion Criteria:

  • healthy, older than 18

Exclusion Criteria:

  • non healthy, younger than 18
Both
18 Years and older
Yes
Contact information is only displayed when the study is recruiting subjects
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NCT00801372
0522-08- HMO-CTIL
No
Hadassah Medical Organization
Hadassah Medical Organization
Not Provided
Not Provided
Hadassah Medical Organization
October 2013

ICMJE     Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP