Now Available for Public Comment: Notice of Proposed Rulemaking (NPRM) for FDAAA 801 and NIH Draft Reporting Policy for NIH-Funded Trials

Unrelated Cord Blood Transplant Plus a Haplo-Identical (Half-Matched), T-Cell Depleted Stem Transplant From a Related Donor for Subjects With High Risk Malignancies

This study has been completed.
Sponsor:
Collaborator:
Miltenyi Biotec GmbH
Information provided by (Responsible Party):
Joanne Kurtzberg, MD, Duke University Medical Center
ClinicalTrials.gov Identifier:
NCT00673114
First received: December 27, 2007
Last updated: July 30, 2014
Last verified: July 2014

December 27, 2007
July 30, 2014
August 2007
April 2012   (final data collection date for primary outcome measure)
The Number of Participants Reaching Primary Endpoint of Absolute Neutrophil Count (ANC) of 500/uL (Engraftment). [ Time Frame: By day 100 ] [ Designated as safety issue: Yes ]
The primary endpoint of the study is number of days to ANC of 500/uL [ Time Frame: By day 100 ] [ Designated as safety issue: Yes ]
Complete list of historical versions of study NCT00673114 on ClinicalTrials.gov Archive Site
  • 180 Day Survival [ Time Frame: 180 days ] [ Designated as safety issue: No ]
    Number of participants alive at 180 days post transplant
  • Non-Relapse Mortality at 180 Days Post Transplant [ Time Frame: 180 days ] [ Designated as safety issue: No ]
  • Platelet Engraftment (Untransfused and Platelet Count > 50,000) [ Time Frame: Approximately 1 year ] [ Designated as safety issue: No ]
    Participants platelet engrafted.
  • Incidence of Primary and Secondary Graft Failure [ Time Frame: 100 days post transplant ] [ Designated as safety issue: No ]
    Number of participants experiencing graft failure.
  • Number of Participants With Acute or Chronic Graft-versus-host Disease (GVHD) [ Time Frame: two years ] [ Designated as safety issue: No ]
    Acute and chronic GVHD
  • Rates of Leukemic Relapse [ Time Frame: Up to 2 years post transplant ] [ Designated as safety issue: No ]
    Number of participants relapsed
  • Number of Participants With Donor Cells at 100 Days Post-transplant [ Time Frame: Post transplant ] [ Designated as safety issue: No ]
  • 180 day survival [ Time Frame: 180 days ] [ Designated as safety issue: No ]
  • Non-relapse mortality in the first 180 days post transplant [ Time Frame: 180 days ] [ Designated as safety issue: No ]
  • Number of days to untransfused platelet count of 50K/uL [ Time Frame: Approximately 1 year ] [ Designated as safety issue: No ]
  • Incidence of primary and secondary graft failure [ Time Frame: 100 days post transplant ] [ Designated as safety issue: No ]
  • Incidence and severity of acute and chronic graft-versus-host disease (GVHD) [ Time Frame: acute- through 100 days, chronic- indefinitely ] [ Designated as safety issue: No ]
  • Pace and quality of immune reconstitution [ Time Frame: 1 year ] [ Designated as safety issue: No ]
  • Rates of leukemic relapse [ Time Frame: 1 year and beyond ] [ Designated as safety issue: No ]
  • Donor chimerism [ Time Frame: Measured around day 28, 42, 60, 100, 180, 270, 360 and yearly (at minimum) ] [ Designated as safety issue: No ]
Not Provided
Not Provided
 
Unrelated Cord Blood Transplant Plus a Haplo-Identical (Half-Matched), T-Cell Depleted Stem Transplant From a Related Donor for Subjects With High Risk Malignancies
A Prospective, Phase I/II Trial Determining the Efficacy and Safety of Allogeneic Hematopoietic Stem Cell Transplantation Using Banked Unrelated Umbilical Cord Blood Supplemented With Related, Haplo-Identical T-Cell Depleted Stem Cells in Subjects With High Risk Malignancies

Subjects will be diagnosed with a hematological malignancy (cancer of the blood), which is unlikely to be cured with conventional non-transplant therapy. The best results of bone marrow transplant are obtained with the donor is a relative that has identical tissue type (HLA-type). These subjects will not have such a donor available but they will have a appropriately matching unrelated umbilical cord blood unit (UCB). However, the cord blood unit does not contain a high enough number of cells and may take longer to engraft (or grow). The purpose of this study is to determine whether the addition of stem cells from a family member to supplement a standard unrelated cord blood transplant is safe and will increase the success of the cord blood transplantation procedure. Subjects enrolled in this study will receive an unrelated cord blood transplant plus a haplo-identical (half-matched), T-cell depleted stem transplant from a related donor. The goal of this study is to determine whether the addition of the related stem cells accelerates bone marrow recovery and improves long-term disease free survival.

Over the past decade, umbilical cord blood transplantation has been shown to be a viable alternative donor stem cell source for hematopoietic cell transplantation in subjects with catastrophic diseases treatable with transplantation therapy. UCB cells can cross partially mismatched HLA barriers without intolerable acute or chronic Graft-versus-Host Disease(GVHD). Thus, many subjects lacking a sufficiently matched, living related or unrelated bone marrow or adult stem cell donor, can use partially HLA-matched UCB cells for stem cell rescue after myeloablative irradiation and/or chemotherapy. UCB Cell dose, expressed per kilogram of recipient body weight, is the best predictor of outcomes after UCB transplantation. Cell dose thresholds strongly correlating with outcomes have been identified.

In subjects receiving lower cell doses, while durable engraftment will ultimately occur, there are significant delays in myeloid and platelet engraftment which, at best, result in longer hospitalization and significant increases in resource utilization and in the worst cases, result in increased early deaths from infection and regimen-related toxicity.

In infants and children weighing <40kg, it is possible to find a sufficiently matched UCB unit that will deliver a dose of cells critical for successful engraftment (defined as 5 x 10^7 nucleated cells/kg) within a reasonable time frame in >90% of subjects. In teenagers and adults weighing >40kg, this is not always possible. Because UCB units contain a relatively fixed number of total nucleated cells, units delivering optimal cell dosing for subjects weighing >70kg will only be identified <10% of the time. Attempts to increase the dose of cells available for UCBT have included ex vivo expansion and combined unit transplantation. While expansion of UCB cells ex vivo is possible, infusion of these expanded cells have not resulted in shortening of engraftment times. Likewise, combinations of up to 5 UCB units for a single myeloablative transplant have not shortened time to neutrophil or platelet engraftment.

In this study, we take an alternative approach to facilitating early myeloid engraftment in subjects undergoing UCB transplantation therapy. In subjects who cannot only identify a donor delivering a cell dose >2 x 10^7 nucleated cells/kg, we will augment the UCBT with a lower dose of haplo-identical, T-cell depleted stem cells from a related adult donor to facilitate early, short-term engraftment with the primary goal of minimizing early infections and other non-relapse mortality while the UCB cells engraft as the durable and permanent graft. As the immunocompetent UCB cells engraft, we expect that they will reject the immunologically incompetent haplo-identical adult stem cells. Thus, after approximately 100-180 days post transplant, the subject should convert to 100% donor chimerism with the UCB donor graft.

In this study, we will investigate the use of unrelated UCB obtained from the umbilical cord blood banks supplements with related, haplo-identical, T-cell depleted stem cells in subjects with high risk refractory malignancies, myelodysplasia or severe aplastic anemia amenable to stem cell transplantation therapy but lacking conventional related or unrelated donors.

OBJECTIVES:

  1. To determine the safety of co-transplantation of unrelated umbilical cord blood supplemented with related, haplo-identical, T-cell depleted stem cells in subjects with high risk malignancies.
  2. To describe the rates of neutrophil and platelet engraftment and immune reconstitution in these subjects.
  3. To determine whether short and long term lymphohematopoietic engraftment is derived from one or both donor sources.

The primary endpoint of the study is number of days to ANC of 500/uL

The secondary endpoints of the study are:

  1. 180 day survival
  2. Non-relapse mortality in the first 180 days post transplant
  3. Number of days to untransfused platelet count of 50K/uL
  4. Incidence of primary and secondary graft failure
  5. Incidence and severity of acute and chronic graft-versus-host disease (GVHD)
  6. Pace and quality of immune reconstitution
  7. Rates of leukemic relapse
  8. Donor chimerism
Interventional
Phase 1
Phase 2
Endpoint Classification: Safety/Efficacy Study
Intervention Model: Single Group Assignment
Masking: Open Label
Primary Purpose: Treatment
  • Hematologic Malignancy
  • Myelodysplastic Syndrome (MDS)
  • Aplastic Anemia
Biological: haplo/cord transplant
T-cell depleted haplo-matched cells from related donor and unrelated umbilical cord blood
Transplant Recipients
Intervention: Biological: haplo/cord transplant
Not Provided

*   Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.
 
Completed
3
April 2012
April 2012   (final data collection date for primary outcome measure)

Inclusion Criteria:

  • Patient Selection Criteria: Patients with high risk or refractory malignancies, myelodysplasia (MDS) or severe aplastic anemia amenable to stem cell transplantation therapy but lacking conventional related or unrelated donors will be eligible for this trial.
  • Have a consenting related haplo-identical (3/6 or 4/6) stem cell donor;
  • Have an available 3, 4, 5, or 6/6 antigen matching unrelated UCB unit that will deliver a cell dose between 2.0-5.0 x 10e7cells/kg.
  • Not have a consenting 6/6 or 5/6 antigen matched related bone marrow donor or genetically matched unrelated BM or adult stem cell donor.
  • Patients must be <55 years of age at the time of study enrollment.
  • Patients must have histologically confirmed diagnosis of a hematologic malignancy, MDS or severe aplastic anemia. Eligible patients include the following:
  • Patients with high risk ALL in first complete remission, with high risk being defined by the presence of hypodiploidy, t(4;11; MLL. 11q23) or t(9;22), or patients presenting with extreme hyperleukocytosis (initial WBC >500,000/ml) or failure to achieve a complete remission after standard induction therapy.
  • All patients with ALL or ANLL in second or subsequent remission.
  • Patients with ALL or ANLL in relapse.
  • Patients with MDS.
  • Patients with CML in any chronic phase, accelerated phase or blast crisis.
  • Patients with severe aplastic anemia refractory to medical therapy.
  • Patients must not have active CNS disease at the time of study enrollment.
  • Patients must have a good performance status (Lansky 80-100%, Karnofsky 50-100%).
  • Patients must have adequate function of other organ systems as measured by:
  • Creatinine < 2.0 mg/dl and creatinine clearance > 50 cc/min/m2.
  • Hepatic transaminases (ALT/AST) < 4 x normal, bilirubin < 2.0 mg/dl.
  • Normal cardiac function by echocardiogram or radionuclide scan, (ejection fraction or shortening fraction > 80% of normal value for age).
  • Pulmonary function tests demonstrating FVC and FEV1 of >60% of predicted for age. For adult patients DLCO > 60% of predicted. If patient cannot perform PFTs, clearance by the pediatric or adult pulmonologist will be required.
  • Patients must not have uncontrolled infections at the time of cytoreduction.
  • Patient, parent, or legal guardian must have given written informed consent according to FDA guidelines.
  • Patients may not be pregnant or lactating and must have a current negative pregnancy test.
  • Patients must have a minimum life expectancy of at least 3 months.
  • Patients must have an available related haplo-identical stem cell donor and an available unrelated cord blood donor delivering between 2 x10e7 cells/kg and 5 x 10e7 cells/kg and matching at a minimum of 3/6 HLA loci.
  • Patients must be HIV negative.
  • Patients must not be concurrently involved in any other clinical trial that affects engraftment or immune reconstitution (e.g. other hematopoietic growth factors).
  • Patients must not have any co-morbid condition which, in the view of the Principal Investigators, renders the patient at too high a risk from treatment complications and regimen related morbidity/mortality.
Both
up to 55 Years
No
Contact information is only displayed when the study is recruiting subjects
United States
 
NCT00673114
Pro00008292, 8717
Yes
Joanne Kurtzberg, MD, Duke University Medical Center
Joanne Kurtzberg, MD
Miltenyi Biotec GmbH
Principal Investigator: Joanne Kurtzberg, MD Duke University Medical Center Pediatric Blood and Marrow Transplant
Duke University
July 2014

ICMJE     Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP