Exercise Changes to Peripheral Blood Mononuclear Cells in Children

This study is currently recruiting participants. (see Contacts and Locations)
Verified November 2010 by University of California, Irvine
Sponsor:
Information provided by:
University of California, Irvine
ClinicalTrials.gov Identifier:
NCT00585403
First received: December 26, 2007
Last updated: November 5, 2010
Last verified: November 2010

December 26, 2007
November 5, 2010
September 2004
January 2015   (final data collection date for primary outcome measure)
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Complete list of historical versions of study NCT00585403 on ClinicalTrials.gov Archive Site
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Exercise Changes to Peripheral Blood Mononuclear Cells in Children
PBMC, Exercise and Children: Initial Mechanisms

The goal of this research is to determine how the peripheral immune system is altered by exercise and differences related to gender, pubertal status and health.

SPECIFIC AIMS:

  1. To systematically measure for the first time in healthy children and adolescents the effects of brief bouts of exercise on:

    1. Numbers of circulating PMBCs, their subsets and key intercellular adhesion molecules (ICAMs).
    2. PBMC gene regulation of stress, inflammatory, and growth/repair mediators [including: interleukin-4 (IL-4), IL-6, IL-10, tumor necrosis factor-alpha (TNF-alpha), interferon-gamma), growth hormone (GH), insulin-like growth factor-I (IGF-I), heat shock proteins (Hsp)].
    3. Circulating (serum) and intracellular PBMC levels of key mediators by flow cytometry and cell culture techniques.
    4. Circulating endogenous triggers of PBMC mediator responses—soluble Hsp, IL-6, and F2-isoprostanes.
  2. To determine how the acute PBMC responses are altered by gender, pubertal status, body composition (measured by whole-body and regional DEXA), and fitness (measured by progressive cycle ergometry and gas exchange).
  3. To determine the relationship in healthy children and adolescents among acute PBMC responses to exercise, biochemical precursors of the metabolic syndrome (insulin, glucose, lipids), and the balance of the TH1/TH2 immune response.
Observational
Observational Model: Cohort
Time Perspective: Cross-Sectional
Not Provided
Retention:   Samples With DNA
Description:

Peripheral blood mononuclear cells, plasma, serum, mRNA

Non-Probability Sample

Early and late pubertal boys and girls recruited from Orange County, CA

  • Physical Fitness
  • Obesity
  • Child Development
  • Growth
Not Provided
Children
Early and late pubertal girls and boys
Not Provided

*   Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.
 
Recruiting
160
February 2015
January 2015   (final data collection date for primary outcome measure)

Inclusion Criteria:

  • appropriate Tanner Stage
  • No evidence of disease or disability

Exclusion Criteria:

  • no use of antiinflammatory medications, alcohol, illegal drugs or bronchodilators
  • elite children participating in extensive exercise or dance programs
  • pregnant
Both
8 Years to 18 Years
Yes
Contact: Lori D. Wilson, PhD 714-456-2246 lwilson@uci.edu
United States
 
NCT00585403
PBMC Exercise, HL-80947
Yes
Dan M. Cooper, UC Irvine, Dept of Pediatrics
University of California, Irvine
Not Provided
Principal Investigator: Dan M Cooper, M.D. Univ. California, Irvine, CA
University of California, Irvine
November 2010

ICMJE     Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP