Gene Message (mRNA) Analysis of Granulocytes

This study is currently recruiting participants.
Verified January 2014 by National Institutes of Health Clinical Center (CC)
Sponsor:
Information provided by:
National Institutes of Health Clinical Center (CC)
ClinicalTrials.gov Identifier:
NCT00059423
First received: April 25, 2003
Last updated: March 14, 2014
Last verified: January 2014

April 25, 2003
March 14, 2014
April 2003
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Complete list of historical versions of study NCT00059423 on ClinicalTrials.gov Archive Site
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Gene Message (mRNA) Analysis of Granulocytes
Natural History and Molecular Characterization of Benign Ethnic Neutropenia in Individuals of African Descent

In recent decades, hematologists have noticed that persons of African descent sometimes have lower white blood cell counts of a certain type, called granulocytes. These cells help to fight infections. The lower number of granulocytes in this situation does not appear to lead to more infections, and these individuals do not have any symptoms. This condition is called benign ethnic neutropenia (BEN), and is observed in a small percentage of individuals of African descent. This study will investigate the condition by studying people with and without BEN.

The goals of this study are to:

  1. identify individuals of African descent with BEN.
  2. determine the effects of two drugs, G-CSF and dexamethasone, on granulocyte production and movement.
  3. determine whether there are differences in those with and without BEN in the way genes are stimulated after the administration of G-CSF and dexamethasone.

Study participants will be asked to interview with the research team, undergo physical exams, donate a blood sample, and receive G-CSF by injection, followed by dexamethasone (orally) about three weeks later. They also will be required to undergo apheresis three times, a procedure in which blood is drawn from a donor and separated into its components. Some components are retained for research analyses, such as granulocytes, and small amount of blood; the remainder is returned by transfusion to the donor. This procedure will be required of participants before they receive G-CSF, the day after they receive G-CSF, and the day after they receive dexamethasone. Gene messages (mRNA will be isolated from granulocytes, and analyzed to better understand granulocyte growth and movement.

Benign ethnic neutropenia (BEN) is defined by peripheral blood absolute neutrophil count less than 1.5 x 10 (9) per liter without an increase in infections. This condition has been described in individuals of African descent. Although these individuals have normal myeloid maturation on bone marrow examinations, they appear to release fewer neutrophils into the circulation when stimulated by hydrocortisone, compared to normal controls. This suggests that there may be differences in the regulation of neutrophil release or trafficking. In the past decade, granulocyte-colony stimulating factor (G-CSF) has been widely used in a variety of clinical settings, from patients with chemotherapy-induced neutropenia to normal volunteers for peripheral blood stem cell collection. G-CSF, however, has not been used in individuals with BEN. Furthermore, gene expression in neutrophil proliferation and trafficking has not been studied in these individuals. The purposes of this study are to (1) identify individuals with BEN; (2) follow the natural history of BEN; (3) determine if there is a familial inheritance pattern; (4) characterize and compare neutrophil response to dexamethasone and G-CSF; (5) compare the pattern of neutrophil gene expression by microarray analyses; and (6) determine if mutations are present at the DNA level to account for gene expression pattern differences in individuals of African descent with and without BEN at baseline, post dexamethasone, and post G-CSF stimulation.

Observational
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Neutropenia
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*   Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.
 
Recruiting
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  • INCLUSION CRITERIA:
  • Individuals of African descent of age 5 or greater
  • Normal renal function: creatinine < 1.5 mg/dL and proteinuria < 1+
  • Normal liver function: bilirubin < 1.5 mg/dL and transaminases within normal limits
  • For control subjects: WBC within normal range (3,300-9,600/mm3), granulocytes (Bullet)1,500/mm3, platelets > 150,000/mm3, hemoglobin > 11.5g/dL and normal MCV
  • For benign ethnic neutropenic subjects: two blood counts, at least 1 month apart, in the last 6 months, with granulocytes < 1,500/mm3, platelet > 150,000/mm3, hemoglobin > 12.5g/dL, and normal MCV
  • Female volunteers of childbearing age should not be pregnant
  • Meets NIH Department of Transfusion Medicine (DTM) eligibility criteria for blood component donation for in vitro research uses (negative serologic tests for syphilis, hepatitis B and C, HIV, and HTLV-1)
  • Ability to give informed consent to participate in the protocol

EXCLUSION CRITERIA:

  • Any underlying hematologic disorder including anemia, and sickle cell disease. Subjects with thalassemia or sickle cell trait are not excluded.
  • Current use of corticosteroids, e.g. prednisone, dexamethasone, or hydrocortisone. Corticosteroids must be discontinued at least one month prior
  • Active or chronic viral, bacterial, fungal, or parasitic infection. Any antibiotic use should be discontinued at least one month prior

4.2.4< TAB> History of autoimmune disease, such as rheumatoid arthritis or systemic lupus erythematosus, or positive anti-nuclear antibody (ANA ELISA) of 3 E.U. (ELISA units) or greater.

  • Low B12 or folate levels, or abnormal thyroid function tests
  • History of cancer or chemotherapy, except squamous carcinoma of the skin and cervical carcinoma in situ
  • Pregnant woman or positive urine pregnancy test
  • History of clinically significant cardiovascular disease (cardiology consultation may be obtained when clinically indicated)
  • Any positive serum screening test as listed below
  • Allergy to G-CSF or bacterial E. coli products
  • Active pulmonary disease or a pulse-ox level of less than 95% on screening exam
Both
5 Years and older
Yes
Contact: Matthew M Hsieh, M.D. (301) 402-7687 matthewhs@mail.nih.gov
United States
 
NCT00059423
030168, 03-H-0168
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National Heart, Lung, and Blood Institute (NHLBI)
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Principal Investigator: Matthew M Hsieh, M.D. National Heart, Lung, and Blood Institute (NHLBI)
National Institutes of Health Clinical Center (CC)
January 2014

ICMJE     Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP