Cold Stress Stimulate the Browing of Subcutaneous White Adipose in Healthy Adults

This study is currently recruiting participants. (see Contacts and Locations)
Verified June 2014 by Wuhan General Hospital of Guangzhou Military Command
Information provided by (Responsible Party):
Xiang Guang-da, Wuhan General Hospital of Guangzhou Military Command Identifier:
First received: May 31, 2014
Last updated: June 13, 2014
Last verified: June 2014

Adipose tissues, which include white adipose tissue (WAT) and brown adipose tissue (BAT), play an essential role in regulating whole-body energy homeostasis. Recent studies also reveal the presence of a subset of cells in WAT that could be induced by environmental or hormonal factors to become ''brown-like'' cells, and this ''beigeing'' process has been suggested to have strong antiobesity and antidiabetic benefits. More recently, same studies showed that cold stress stimulate the browning of subcutaneous white adipose. However, to the investigators knowledge, there are no direct data that clearly show that cold stress can promote the browning of subcutaneous white adipose.

Condition Intervention
Type 2 Diabetes
Other: cold stress

Study Type: Interventional
Study Design: Intervention Model: Single Group Assignment
Masking: Open Label
Official Title: Cold Stress Stimulate the Browing of Subcutaneous White Adipose in Healthy Adults

Resource links provided by NLM:

Further study details as provided by Wuhan General Hospital of Guangzhou Military Command:

Primary Outcome Measures:
  • browning of subcutaneous white adipose [ Time Frame: 4 months ] [ Designated as safety issue: No ]
    Brown fat characteristics of biopsy samples will be assessed by determining the expression levels of UCP1, PGC1a, PRDM16 and Grb10.

Estimated Enrollment: 10
Study Start Date: June 2014
Estimated Study Completion Date: September 2014
Estimated Primary Completion Date: September 2014 (Final data collection date for primary outcome measure)
Arms Assigned Interventions
Experimental: healthy adults Other: cold stress

Detailed Description:

Individuals have cold exposure at 15C for 2 hr everyday for one week.Biopsy for subcutaneous white adipose was performed before and after one week cold exposure programme under local anesthesia. Measure the brown fat characteristics of biopsy samples. The expression levels of uncoupling protein-1 (UCP-1), peroxisome proliferator-activated receptor (PPAR)-r, peroxisome proliferator-activated receptor r coactivator 1 a (PGC1a) , growth factor receptor binding protein-10 (Grb10), PR domain containing 16 (PRDM16) will be determined before and after cold stress programme from the biopsy samples. In addition, a combination of PET and computed tomography (CT) — with the glucose analogue 18F-fluorodeoxyglucose (18F-FDG) as a tracer will be performed for brown adipose tissue before and after cold stress programme.


Ages Eligible for Study:   20 Years to 60 Years
Genders Eligible for Study:   Male
Accepts Healthy Volunteers:   Yes

Inclusion Criteria:

  • healthy men
  • 18-60 years old
  • BMI 20-28 kg/m2

Exclusion Criteria:

  • BMI < 20 kg/m2
  • BMI > 28 KG/M2
  • Female
  Contacts and Locations
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the Contacts provided below. For general information, see Learn About Clinical Studies.

Please refer to this study by its identifier: NCT02159144

Contact: Xiang Guangda, MD, phD +8602768878411

China, Hubei
Wuhan General Hospital Recruiting
Wuhan, Hubei, China, 430070
Contact: Xiang Lin, MD    02768879059   
Sponsors and Collaborators
Xiang Guang-da
  More Information

No publications provided

Responsible Party: Xiang Guang-da, Director of Endocrinology Department, Wuhan General Hospital of Guangzhou Military Command Identifier: NCT02159144     History of Changes
Other Study ID Numbers: 2014Wze088
Study First Received: May 31, 2014
Last Updated: June 13, 2014
Health Authority: China: the ethic committee of Wuhan General Hospital

Keywords provided by Wuhan General Hospital of Guangzhou Military Command:
Brown adipose tissue
Subcutaneous white adipose tissue
White adipose tissue

Additional relevant MeSH terms:
Diabetes Mellitus, Type 2
Diabetes Mellitus
Glucose Metabolism Disorders
Metabolic Diseases
Endocrine System Diseases
Nutrition Disorders
Body Weight
Signs and Symptoms processed this record on August 18, 2014