Molecular Analysis of Microbial DNA in Infected Necrotizing Pancreatitis
Necrotizing pancreatitis is a severe form of inflammation of the pancreas with subsequent destruction of the pancreas (necrosis). Often, this condition manifests in patients as an overwhelming systemic inflammatory response and multisystem organ failure. Many times the pancreas can become superinfected with bacteria and other organisms as a result of this process. Clinicians may decide to sample fluid from the pancreas or pancreatic tissue to determine the presence of infection. This can be done through surgery with a concurrent debridement and drainage of the pancreas or by a radiologist using an ultrasound or CT guided method to sample the pancreatic fluid. These samples are then sent for culture in the microbiology laboratory. Culture results often reveal 1-2 types of infecting bacteria that originate from the gastrointestinal tract. The investigators hypothesize that in actuality, many different kinds of bacteria may infect the pancreas. This project uses molecular DNA techniques to help identify bacteria present in the pancreas of patients with severe necrotizing pancreatitis.
Bacteria Infecting Necrotizing Pancreatitis
Systemic Inflammatory Response Syndrome
Comparison of Molecular Techniques for Bacterial Identification to Current Standard Culture Techniques
|Study Design:||Observational Model: Case Control
Time Perspective: Prospective
|Official Title:||Molecular Analysis of Microbial DNA in Infected Necrotizing Pancreatitis|
- Bacteria [ Time Frame: June 2012 ] [ Designated as safety issue: No ]Bacteria present in peri-pancreatic fluid and pancreatic tissue will be analyzed and identified suing molecular based methods. Comparisons will be made to bacteria identified using standard culture techniques.
- Cytokines [ Time Frame: June 2012 ] [ Designated as safety issue: No ]Blood samples will be taken from each patient to measure the level of different cytokines at the time of sample collection
- Gastrointestinal Bacterial Flora [ Time Frame: June 2012 ] [ Designated as safety issue: No ]A small stool sample will be taken from each patient to determine normal GI flora that may be translocating to the pancreas in patients with severe pancreatitis.
Biospecimen Retention: Samples With DNA
Peripancreatic fluid, pancreatic tissue, blood sample, stool sample
|Study Start Date:||July 2011|
|Estimated Study Completion Date:||June 2014|
|Estimated Primary Completion Date:||June 2014 (Final data collection date for primary outcome measure)|
|Contact: Erin M Hanna, MDfirstname.lastname@example.org|
|Contact: Sherrie B Lee, RN, BSNemail@example.com|
|United States, North Carolina|
|Carolinas Medical Center||Recruiting|
|Charlotte, North Carolina, United States, 28204|
|Contact: Erin M Hanna, MD 704-355-1162 firstname.lastname@example.org|
|Principal Investigator: David Sindram, MD, PhD|
|Principal Investigator:||David Sindram, MD||Carolinas Healthcare System|