Effects of Phototherapy by Low-level Light-emitting Diode Therapy (LEDT) on Clinical, Biochemical, Biomechanical, Histologic and Genetic Responses of Muscle Performance of Young Males Submitted to Physical Strength Training

This study has been completed.
Sponsor:
Collaborators:
Fundação de Amparo à Pesquisa do Estado de São Paulo
Centro de Pesquisa em Óptica e Fotônica de São Carlos (CePOF)
Information provided by (Responsible Party):
Cleber Ferraresi, Universidade Federal de Sao Carlos
ClinicalTrials.gov Identifier:
NCT01770938
First received: September 15, 2012
Last updated: January 15, 2013
Last verified: September 2012
  Purpose

Muscle performance is largely influenced by modulations in gene expressions of muscle tissue. In this context, light-emitting diode therapy (LEDT) has been used to improve muscle performance in experimental models and human researches. Thereby, the investigators examined modulations in gene expression of muscle tissue as well as biochemical, biomechanic and histologic adaptations influenced by exercise associated to LEDT.


Condition Intervention
Healthy
Device: Effective light-emitting diode therapy
Device: Placebo light-emitting diode therapy

Study Type: Interventional
Study Design: Allocation: Randomized
Endpoint Classification: Efficacy Study
Intervention Model: Crossover Assignment
Masking: Double Blind (Subject, Investigator)
Primary Purpose: Treatment
Official Title: Effects of Phototherapy by Low-level Light-emitting Diode Therapy (LEDT) on Clinical, Biochemical, Biomechanical, Histologic and Genetic Responses of Muscle Performance of Young Males Submitted to Physical Strength Training

Further study details as provided by Universidade Federal de Sao Carlos:

Primary Outcome Measures:
  • Modulations on muscle gene expression by RT-PCR [ Time Frame: 12 weeks ] [ Designated as safety issue: No ]

Secondary Outcome Measures:
  • Muscle performance in leg press device [ Time Frame: 12 weeks ] [ Designated as safety issue: No ]
  • Muscle performance in leg extension device [ Time Frame: 12 weeks ] [ Designated as safety issue: No ]
  • Muscle performance in isokinetic dynamometer [ Time Frame: 12 weeks ] [ Designated as safety issue: No ]
  • Gene expression [ Time Frame: 12 weeks ] [ Designated as safety issue: No ]
    Gene expression of muscle tissue by RT-PCR


Other Outcome Measures:
  • Immunohistochemical analysis [ Time Frame: 12 weeks ] [ Designated as safety issue: No ]
    Immunohistochemical analysis to quantify morphology and cells of muscle tissue as satellite cells.


Enrollment: 32
Study Start Date: May 2012
Study Completion Date: September 2012
Primary Completion Date: July 2012 (Final data collection date for primary outcome measure)
Arms Assigned Interventions
Active Comparator: Effective light-emitting diode therapy and training
Effects of effective light-emitting diode therapy therapy on muscle performance of young males submitted to physical strength training
Device: Effective light-emitting diode therapy
Other Names:
  • LED
  • LED therapy
  • Light-emitting diode therapy
Placebo Comparator: Placebo light-emitting diode therapy and training
Effects of placebo light-emitting diode therapy on muscle performance of young males submitted to physical strength training
Device: Placebo light-emitting diode therapy
Other Names:
  • LED
  • LED therapy
  • Light-emitting diode therapy

Detailed Description:

This study assessed muscle performance in isokinetic dynamometry and led press device, gene expression by RT-PCR, lactate, creatine kinase and morphology of muscle tissue by immunohistochemical analysis

  Eligibility

Ages Eligible for Study:   18 Years to 28 Years
Genders Eligible for Study:   Male
Accepts Healthy Volunteers:   Yes
Criteria

Inclusion Criteria:

  • healthy males aged between 18 and 28 years with a body mass index (BMI) equal to or less than 26 kg/m2,
  • moderated pattern of physical activity

Exclusion Criteria:

  • previous injury to the femoral quadriceps or hamstring muscles (within 6 months prior to study),
  • osseous or articular disorder in the lower limbs,
  • cardiovascular system disorders,
  • systemic disease, and
  • taking prescription medicines or using dietary supplements such as muscle mass builders.
  Contacts and Locations
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the Contacts provided below. For general information, see Learn About Clinical Studies.

Please refer to this study by its ClinicalTrials.gov identifier: NCT01770938

Locations
Brazil
Federal University of São Carlos
São Carlos, São Paulo, Brazil, 13565-905
Sponsors and Collaborators
Universidade Federal de Sao Carlos
Fundação de Amparo à Pesquisa do Estado de São Paulo
Centro de Pesquisa em Óptica e Fotônica de São Carlos (CePOF)
Investigators
Principal Investigator: Cleber Ferraresi, MSc Universidade Federal de Sao Carlos
Study Director: Nivaldo A Parizotto, PhD Universidade Federal de Sao Carlos
Study Director: Vanderlei S Bagnato, PhD University of São Paulo
  More Information

Publications:
Responsible Party: Cleber Ferraresi, MSc, Universidade Federal de Sao Carlos
ClinicalTrials.gov Identifier: NCT01770938     History of Changes
Other Study ID Numbers: CAAE: 0326.0.135.000-11
Study First Received: September 15, 2012
Last Updated: January 15, 2013
Health Authority: Brazil: National Committee of Ethics in Research

Keywords provided by Universidade Federal de Sao Carlos:
Light-emitting diode therapy
Muscle performance
Gene expression
Functional performance

ClinicalTrials.gov processed this record on July 08, 2014