Influence of Different Sugary Beverages on Thirst Sensation

• Motivational Ratings [ Time Frame: 1 day ] [ Designated as safety issue: No ]

  Participants will rate their hunger, thirst, nausea, mouth dryness, desire to eat, desire to drink using nine point category scales. The unipolar adjective scales were anchored at each end with labels: 1 = not at all and 9 = extremely.

  Participants also rated their liking for the beverage along nine point hedonic preference scales where 1 = dislike extremely and 9 = like extremely.

  
  

• Glycaemia [ Time Frame: 1 day ] [ Designated as safety issue: Yes ]
  Glycaemia will be assessed before and after the ingestion of beverages
  
  
• Amount of water ingested [ Time Frame: 1 day ] [ Designated as safety issue: No ]
  After the ingestion of 3 preloads with the beverages in study, the participants will lunch the same meal with free water ingestion.
  
  
• Food Behavior After Intervention [ Time Frame: 1 day ] [ Designated as safety issue: No ]
  After lunch, participants will bring a food diary to record all food and fluid intake until 00.00 that day. Then, we will analyse total calories ingested, total fluid ingested and the volume of sugar and sugary beverages ingested.
  
  
• Blood osmolality [ Time Frame: 1 day ] [ Designated as safety issue: Yes ]
  Blood osmolality will be assessed before and after the ingestion of beverages
  
  
• Plasmatic Sodium [ Time Frame: 1 day ] [ Designated as safety issue: Yes ]
  Plasmatic sodium will be measured before and after the ingestion of the beverages.
  
  
• Hydration parameters [ Time Frame: 1 day ] [ Designated as safety issue: Yes ]
  Total body water, intra and extracellular body water will me measured before and after the ingestion of the beverages
  
  

Participants should be in the laboratory at 8.00 in order to collect the values of weight, height, total body water, intracellular water and extracellular water in a Segmental Multi Frequency Body Composition Monitor (TANITA MC 180 MA®). Then, a blood sample will be collected for analysis of blood glucose, osmolality and plasmatic sodium. They will sit at individual tables for the duration of the session and were allowed to read, listen to music with earphones, or use their portable computers with the exception of internet access to minimize visual cues (e.g., unwanted publicity for beverages or visiting Web sites showing pictures of food and drinks), which may have effect on thirst. Breakfast will be served at 9.30 on every occasion and preload stimuli will be offered exactly 60 minutes after breakfast (at 10.30). Lunch will be provided at 13.00. Motivational ratings will be first obtained at the end of collection of blood sample (baseline or time 0) and every 30 minutes thereafter until the lunch time (times 1 through 7). After lunch (time 8), participants will complete the last set of ratings and will bring a food diary to record all food and fluid intake until 00.00 that day.