Influence of Preconceptional Obesity on the Myometrial Cells Differentiation Toward a Contractyl Phenotype (ObeDi)
The purpose of this study is to improve the understanding of cellular mecanism involved in delivery disorders in obese women, first by characterizing the hyperplasia phenomenon of myometrail cells induced by inflammatory stimulation (Il-1B), then by studying the role of blood mononuclear cells, of leptine and of obese women sera to interfere with the transition of myometrial cells toward hyperplasia phenotype.
|Study Design:||Intervention Model: Single Group Assignment
Masking: Open Label
Primary Purpose: Basic Science
|Official Title:||Study of Role of Mononuclear Cells of Maternal Peripheric Blood in Human Myometrial Primar Cell Lines Differentiation Toward a Contratyl Phenotype: Influence of Preconceptional Obesity|
- Measuring the expression level of markers (Gama actine, Desmine, Connexine-43, and Ocytocine receptor) by Westernblotting [ Time Frame: up to 2 months ] [ Designated as safety issue: No ]Demonstrate in vitro the capacity of a Il-1B stimulus to induce the differentiation of the myometrial cells toward a hyperplasia phenotype, and that the answer depends on whether this is cells from normal weight women or obese women before conception.
|Study Start Date:||April 2012|
|Estimated Primary Completion Date:||November 2013 (Final data collection date for primary outcome measure)|
Other: Myometrium biopsy and blood samples
The biopsy of myometrium is specially realised for the study in surgical unit after the release of the child, in women with normal pregnancy but necessitating a caesarean.
The blood samples are realised, specially for the study, either during the medical exam before the ceasarean or specifically for the study.
|Contact: Marc BARDOU, Pr||380-393-433 ext +33||Marc.Bardou@u-bourgogne.fr|
|Maternité du CHU de Dijon||Recruiting|
|Dijon, Bourgogne, France, 21000|
|Contact: Marc BARDOU, Pr 380-393-433 ext +33 firstname.lastname@example.org|
|Contact: Isabelle LE RAY, Md 380-393-223 ext +33 Isabelle.Le-Ray-Ferrieres@u-bourgogne.fr|
|Principal Investigator: Paul SAGOT, Pr|