The Effect of Folic Acid Supplementation and Pregnancy on the Folate Forms in Red Blood Cells
The purpose of this cross-sectional study was to examine whether or not physical changes or adaptations occur in response pregnancy or to the higher intakes of folic acid from supplementation typically consumed during the reproductive period.
|Study Design:||Observational Model: Cohort
Time Perspective: Cross-Sectional
|Official Title:||The Effect of Folic Acid Supplementation and Pregnancy on the Folate Forms in Red Blood Cells|
- Red blood cell folate concentrations - tetrahydrofolate (THF) [ Time Frame: At the point of participation (this is a single visit, single test study) ] [ Designated as safety issue: No ]Bood samples were taken once at the point of participation and compared 1) pregnant women (between 30-36 weeks gestation) and non-pregnant women who were taking supplements 2) 0 mg folic acid 3) 1 mg FA, 4) and 5 mg FA. Those who were taking supplements were taking them for a minimum of 30 weeks.
- Red blood cell folate concentrations - 5-methyltetrahydrofolate (5-methylTHF) [ Time Frame: At the point of participation (this is a single visit, single test study) ] [ Designated as safety issue: No ]
- Red blood cell concentrations - 5-formyltetrahydrofolate (5-formylTHF) [ Time Frame: At the point of participation (this is a single visit, single test study) ] [ Designated as safety issue: No ]
- Red blood cell folate concentrations - 5,10-Methenyltetrahydrofolate (5,10-methenylTHF) [ Time Frame: At the point of participation (this is a single visit, single test study) ] [ Designated as safety issue: No ]
|Study Start Date:||May 2008|
|Study Completion Date:||April 2010|
|Primary Completion Date:||October 2008 (Final data collection date for primary outcome measure)|
pregnant women taking 1 mg folic acid;
Dietary Supplement: folic acid
subjects take multivitamin supplement containing folic acid. Women were enrolled based on whether or not they were taking supplements.
non-pregnant women taking 0mg folic acid;
non-pregnant women 2
non-pregnant women taking 1 mg folic acid
non-pregnant women 3
non-pregnant women taking 5 mg folic acid
Higher folate requirements during pregnancy to support growth and development are well established but it is unknown what metabolic changes occur in 1-carbon metabolism to provide sufficient nucleotides for DNA/RNA synthesis. Periconceptual folic acid (FA) supplementation and fortification of the food supply have been shown to reduce the risk of pregnancies with neural tube defects but conversely expose women to high FA intakes for extended periods of time. The purpose of this cross-sectional study is to establish if physiological adaptations occur in folate metabolism in response to pregnancy or higher intakes of FA typically consumed among women during the reproductive period. A convenience sample of thirty-two women (n=8/group) were recruited to compare the total concentrations and forms of folate (tetrahydrofolate [THF], 5-methylTHF, 5-formylTHF and 5, 10-methenylTHF) in red blood cells in four groups: a) pregnant women (PW, 30-36 weeks gestation) taking 1.0 mg of FA b) non-pregnant women (NPW) taking 0 mg of FA c) NPW taking 1.0 mg FA and d) NPW taking 5.0 mg FA. Blood samples were taken at a single time point and compared across groups. Total red blood cell concentrations were measured using both microbiological assay and liquid chromatography/tandem mass spectrometry (LC/MS/MS) analysis. LC/MS/MS analysis was used to determine folate form concentrations.
|The Hospital for Sick Children|
|Toronto, Ontario, Canada, M5G 1X8|
|Principal Investigator:||Deborah L O'Connor, PhD||The Hospital for Sick Children|