Investigation of Stem Cells in Human Lipoaspirate
The overall purpose of this study is to enhance identification and isolation of ADSCs and adipocytes, to find the optimum culturing conditions to increase growth and volume of grafts, and to study the effects of cryopreservation on ADSCs and adipocytes. Fat injections are used in aesthetic surgery but tend to disassemble over time. If the investigators can enhance growth and volume of fat in culture, the investigators could use fat grafts in reconstruction of cancer and traumatic injury defects with the patients own cultured fat cells and grafts.
|Study Design:||Observational Model: Case-Only
Time Perspective: Cross-Sectional
|Official Title:||Investigation of Adipose-Derived Stem Cells in Human Lipoaspirate|
- Percent of Viable ADSCs [ Time Frame: Day of Surgery ] [ Designated as safety issue: No ]Percent of viable ADSCs will be measured on fresh samples and samples refrigerated for different times.
- Percent of Apoptotic ADSCs [ Time Frame: Day of Surgery ] [ Designated as safety issue: No ]Percent of apoptotic ADSCs will be measured on fresh samples and on refrigerated samples at 1, 2, 3, and 4 days refrigerated storage.
- Percent of Necrotic ADSCs [ Time Frame: Day of Surgery ] [ Designated as safety issue: No ]Percent of necrotic ADSCs will be measured on fresh samples and on refrigerated samples at 1, 2, 3, and 4 days of refrigerated storage.
Biospecimen Retention: Samples Without DNA
Cells from lipoaspirate maximum of 20 days
|Study Start Date:||March 2011|
|Estimated Study Completion Date:||August 2018|
|Estimated Primary Completion Date:||August 2018 (Final data collection date for primary outcome measure)|
18-30 yrs, 31-50 yrs, 51-70 yrs
Please refer to this study by its ClinicalTrials.gov identifier: NCT01677520
|Contact: William A Zamboni, M.D.||firstname.lastname@example.org|
|Contact: Linda L Stephenson, BS,MT(ASCP)||email@example.com|
|United States, Nevada|
|University of Nevada School of Medicine Patient Care Center||Recruiting|
|Las Vegas, Nevada, United States, 89102|
|Contact: William A Zamboni, M.D. firstname.lastname@example.org|
|Contact: Karen Zamboni, BSRN 702-671-5118 email@example.com|
|Principal Investigator: William A Zamboni, M.D.|
|Principal Investigator:||William A Zamboni, M.D.||UNSOM|
|Study Director:||Wei Z Wang, M.D.||firstname.lastname@example.org|