Telomeres and Reproduction in Women
The purpose of the study is to describe the relationship between leukocyte, granulose cell and polar body telomere length, telomere injury foci (TIFs), polarized light microscopy of oocyte spindles and their frequency in the eggs from older women and associations with aneuploidy.
|Study Design:||Observational Model: Cohort
Time Perspective: Prospective
|Official Title:||Telomeres and Reproduction in Women|
- Telomere length [ Time Frame: 1 year ] [ Designated as safety issue: No ]Describe the relationship between leukocyte, granulose cell and polar body telomere length, telomere injury foci (TIFs), polarized light microscopy of oocyte spindles
- Frequency of telomeres [ Time Frame: 1 year ] [ Designated as safety issue: No ]The Primary outcome frequencies in the eggs from older women and associations with aneuploidy.
Biospecimen Retention: Samples With DNA
Blood, Follicle cells, and or polar body of eggs
|Study Start Date:||June 2009|
|Study Completion Date:||June 2013|
|Primary Completion Date:||June 2013 (Final data collection date for primary outcome measure)|
This is an observational study; there are no cohorts or groups
The chances of conception decrease and miscarriage increase with a women's age, and the locus of reproductive aging is the egg (1). Egg dysfunction affects most women beginning by their late '30's and for many women age related egg dysfunction represents a major cause of infertility. The cause of age related egg dysfunction is poorly understood, but telomere dysfunction may play an important role. Our group has demonstrated that genetic or pharmacologic shortening of telomeres in the eggs of mice, which normally do not exhibit age related egg dysfunction, mimics reproductive aging in women, with cell cycle arrest, apoptosis, spindle dysmorphologies and chromosomal abnormalities (1,2). Moreover, a pilot study the investigators conducted five years ago suggested that telomere length of spare human eggs may predict the outcome of womens' InVitro Fertilization (IVF) cycles (3). Subsequently, other investigators have demonstrated that telomere length of circulating leukocytes predicts miscarriages (4).