Primary Cell Culture of Hepatic Tumorous Cells From Routine Fine-needle Aspiration

• growth speed of cultured cells [ Time Frame: 28 days after plating of cells ] [ Designated as safety issue: No ]
  correlation the growth speed of cells (reach equal or more than 70% growth area of 25 cm2 culture flask) and TNM tumor staging
  
  

• TNM tumor staging [ Time Frame: 6 months after plating of cells ] [ Designated as safety issue: No ]
  change in tumor staging
  
  
• survival of patient [ Time Frame: 6 months after plating of cells ] [ Designated as safety issue: No ]
  correlation the growth speed of cultured cells measured at 28 days after plating (reach equal or more than 70% growth area of 25 cm2 culture flask)and cancer-related death of patient within 6 months after plating of cells
  
  

Fine-needle aspiration (FNA) cytology or biopsy of hepatic tumor is frequently applied in the collection of specimens because it is considered safe, efficacious, accurate and cost effective. Our previous study from small number of patients showed that specimens obtained from FNA had potential to be applied for primary culture of cancer cells. This study was to modified our method for primary culture of both hepatocellular carcinoma cells and cancer-associated fibroblasts in patients with tumor measuring larger than or equal to 3cm. All patients received one session of aspiration for the diagnosis of hepatic tumor. The aspirated specimens were applied for both cytologic and pathologic examinations at first. After then, 10 mL 0.9% NaCl was aspirated by the same needle connected with the same syringe and injected into 15 mL sterilized centrifugation tube. If the tube contained visible specimens, these specimens were sent for primary culture.