Comparing the Efficacy of Urinary and Recombinant Human Chorionic Gonadotropin (hCG) on Oocyte Maturity

• Number of mature oocytes [ Time Frame: Up to1 hour after oocyte retrieval ] [ Designated as safety issue: Yes ]
  evaluation the effect of recombinant hcg on number of oocytes up to 1 hour after oocytes retrieval
  
  

• number of retrieved oocytes per number of aspirated follicles proportion [ Time Frame: up to 1 hour after oocyte retrieval ] [ Designated as safety issue: Yes ]
  Evaluation the effect of recombinant hCG to retrieve oocytes per number of aspirated follicles proportion
  
  
• fertilization rate [ Time Frame: in 1 day after oocyte retrieval ] [ Designated as safety issue: Yes ]
  Evaluation the effect of recombinant hCG on fertilization rate in 1 day after oocyte retrieval
  
  
• implantation rate [ Time Frame: 4 weeks after embryo transfer ] [ Designated as safety issue: Yes ]
  Evaluation the effect of recombinant hCG on implantation rate 4 weeks after embryo transfer
  
  
• OHSS occurrence rate will be compared between three groups [ Time Frame: from embryo transfer day up to pregnancy test ] [ Designated as safety issue: Yes ]
  Evaluation the effect of recombinant hCG on OHSS occurrence rate will be compared between three groups
  
  
• chemical pregnancy rates [ Time Frame: 2 weeks after embryo transfer ] [ Designated as safety issue: Yes ]
  Evaluation the effect of recombinant hCG on chemical pregnancy rates 2 weeks after embryo transfer
  
  
• clinical pregnancy rates [ Time Frame: 4 weeks after embryo transfer ] [ Designated as safety issue: Yes ]
  Evaluation the effect of recombinant hCG on clinical pregnancy rates 4 weeks after embryo transfer
  
  

The study population comprised of all infertile patients aged 20-37 years which underwent of in-vitro fertilization in royan institute, Tehran Iran.

In this study all patients were treated with a long stimulation protocol in which GnRH-analogue (Buserelin subcutaneous, 0.5cc daily) was given as a pre-treatment and recombinant FSH (rFSH) administration was started when pituitary desensitization was confirmed. From the 7th day of stimulation in both groups, daily monitoring of follicle size by ultrasound was performed and the dose of rFSH was adjusted according to the response of each patient. When at least two follicles > 18 mm diameter were seen, ovulation was triggered with hCG and patients allocated into three different study groups as per the following:

1. Group A consisted of 60 women who received recombinant hCG(250 µg Ovitrelle)
2. Group B consisted of 60 women who received recombinant hCG(500 µg Ovitrelle)
3. Group C consisted of 60 patients received 10,000 IU urinary hCG Oocyte retrieval performed about 34-36 hours after hCG administration and then oocytes were assessed for nuclear maturity. Embryos transferred 2-3 days thereafter.

Data collection will be performed by using questionnaire to be filled as per the available records and laboratory results. Data analysis will be done through descriptive and perceptive statistical methods by using SPSS software version 16 for windows.