TMC207 +/- Rifabutin/Rifampin

Inclusion Criteria:

• Aged between 18 and 45 years, extremes included.
• Non tobacco/nicotine using (at least 3 months prior to screening).
• Body Mass Index (BMI, weight in kg divided by the square of height in meters) of 18.0 to <35.0 kg/m^2
• Informed Consent Form (ICF) signed voluntarily before the first trial-related activity.
• Able to comply with protocol requirements.
• Healthy on the basis of a medical evaluation or history that reveals the absence of any clinically relevant abnormality and includes a physical examination, medical history, electrocardiogram (ECG), vital signs, ophthalmologic exam, the results of blood biochemistry, and hematology tests, and a urinalysis carried out at screening (See Section 7.2).
• Subjects will be enrolled in this study only if they have undergone vasectomy/complete hysterectomy, tubal ligation, or other sterilizing procedure, or the subject is a post-menopausal woman for more than two years, or if sexually active subjects agree to use two of the following forms of adequate contraception during the study and for 12 weeks after the final dose: abstinence, condoms with or without spermicide gel, diaphragm with spermicide gel, hormonal or non-hormonal intrauterine device, oral contraceptive pills, and depot progesterone injections. If a subject is usually not sexually active but becomes active, the subject and his or her partner must use two of the listed contraceptive methods.

Exclusion Criteria:

Medical History

• History or evidence of current use of alcohol, barbiturate, amphetamine, recreational, or narcotic drug use, which in the investigator's opinion would compromise subject's safety and/or compliance with the trial procedures.
• Any clinically significant (as deemed by the Principal Investigator) history of acute illness (resolved within 4 weeks of screening), asthma, or presence of cardiovascular, pulmonary, hepatic, renal, hematologic, gastrointestinal (including eating disorders), endocrine, metabolic, immunologic, dermatologic, neurologic, psychological, or psychiatric disease.
• Currently significant diarrhea, gastric stasis, or constipation that in the investigator's opinion could influence drug absorption or bioavailability.
• Any history of significant skin disease such as, but not limited to, rash or eruptions, drug allergies, food allergy, dermatitis, eczema, psoriasis, or urticaria. Subjects with a history of skin disease may be enrolled into the study after consultation with the Sponsor Medical Monitor.
• Previously demonstrated clinically significant allergy or hypersensitivity to any of the excipients of the investigational medication administered in this trial (i.e., rifabutin, rifampin, and TMC207).
• Subjects with QTcB [Bazett correction] interval > 450ms at screening
• Subjects with any other clinically significant Electrocardiogram (ECG) abnormality at screening, such as arrhythmia, ischemia, or evidence of heart failure or with a family history of Long QT Syndrome.
• History or evidence of ophthalmologic diseases except for routine corrected hyperopia, myopia, and presbyopia.
• Recent history (within past 30 days) of vertigo/nausea.

Specific Treatments

• Current use of any azole antifungal agent
• Use of concomitant medication, including over-the-counter products and dietary supplements, without approval from study staff. Subjects will be treated based on symptom presentation, with the exception of medications that affect p450 and 3a metabolic pathways (refer to the MOP for a list of acceptable medications). During outpatient time periods, subjects will be required to discuss with the study staff and receive approval before self-administering any medication. After gaining approval, subjects will also be asked to record any medication taken during outpatient time periods in a provided log.
• Participation in an investigational drug trial within 60 days prior to the first intake of trial medication and during the duration of the study.
• Donation of blood or significant loss of blood within 56 days or plasma donation within 7 days preceding the first intake of trial medication.
• Having received TMC207 in a previous trial.

Based on Laboratory Abnormalities

• Positive HIV-1 or HIV-2 test by Enzyme-linked immunosorbent assay (ELISA) at screening.
• Hepatitis A, B, or C infection (confirmed by hepatitis A antibody IgM, hepatitis B surface antigen, or hepatitis C virus antibody, respectively) at screening.
• A positive urine drug test at screening. Urine will be tested to check the current use of amphetamines, benzodiazepines, cocaine, cannabinoids, and opioids; along with serum alcohol level.

• Subjects with the following laboratory abnormalities at screening as defined by the National Institute of Health (NIH), National Institute of Allergy and Infectious Diseases (NIAID), Division of Microbiology and Infectious Diseases (DMID) Adult Toxicity Table (Appendix C) and in accordance with the normal ranges of the clinical laboratory:

  1. Serum creatinine grade 1 or greater [> 1.0 x Upper limit of lab normal range (ULN)],
  2. Pancreatic lipase grade 1 or greater (> 1.0 x ULN),
  3. Hemoglobin grade 1 or greater (</= 10.5 g/dL),
  4. Platelet count grade 1 or greater (</= 99000/mm^3),
  5. Aspartate aminotransferase (AST) or alanine aminotransferase (ALT) grade 1 or greater (> 1.0 x ULN),
  6. Total bilirubin grade 1 or greater (> 1.0 x ULN),
  7. Creatine kinase grade 1 or greater (>1.0 x ULN),
  8. Troponin grade 1 or greater (1.0 x UNL), or
  9. Any other toxicity grade 2 or above, including: proteinuria (spot urine) > 1+ and gross hematuria. For the second dose of TMC207, any other toxicity grade 3 or above, including: proteinuria (spot urine) > 1+ and gross hematuria