Urinary Steroid Metabolites in Autism
Deviation of hormone formation within nerve cells and nerve system provides autism spectrum disorder and neurodevelopment retardation through interaction of steroids with neurotransmitter-receptors, calcium-channel receptors and genomic interaction via nuclear steroid receptors.
Urinary steroid metabolites will be compared between children with autism spectrum disorder and healthy controls.
Autism Spectrum Disorder
|Study Design:||Observational Model: Cohort
Time Perspective: Prospective
|Official Title:||Investigation of Urinary Steroid Metabolites in Children With Autism Spectrum Disorder|
- difference in hormone formation and androgen precursors [ Time Frame: 3 years ] [ Designated as safety issue: No ]Calculation of hormone formation by comparison of urinary steroid metabolites
- difference in hormone formation in gender and age [ Time Frame: 3 years ] [ Designated as safety issue: No ]Hormone formation depends on age and gender and will be calculated by comparison of urinary steroid metabolites
Biospecimen Retention: Samples Without DNA
noninvasive overnight urine (morning urine)
|Study Start Date:||September 2010|
|Estimated Study Completion Date:||September 2014|
|Primary Completion Date:||July 2011 (Final data collection date for primary outcome measure)|
Boys with autism spectrum disorder, age 5-15 years, diagnosis meets DSM-IV criteria ascertained by ADI-R or ADOS schedule or specialised paediatrician
Girls with autism spectrum disorder, age 5-15 years, diagnosis meets DSM-IV criteria ascertained by ADI-R or ADOS schedule or specialised paediatrician.
Healthy boys, age 5-15 years, mental status assessed by "Marburger Beurteilungsskala zum Asperger-Syndrome" (MBAS).
Healthy girls, age 5-15 years, mental status assessed by "Marburger Beurteilungsskala zum Asperger Syndrome" (MBAS).
Autism spectrum disorder presents many alterations in amino acid metabolism and in neurotransmitter systems (gamma amino acid system, glutamate-glutamine, nicotine-acid, serotonin-system) . Also alteration in gene activation within nerve cells and lymphocyte cultures encloses over 600 genes, affecting a wide field of enzymes , metabolic pathways and hormone formation. Male hormones and their precursors are often increased and their influence to nerve cell growth and spine formation is evident.
The diagnosis of autism spectrum disorder is made in accordance of criteria of the Diagnostic and Statistical Manual of Mental Disorders IV (DSM-IV) of the American Psychiatric Association by an experienced and specialised paediatrician or psychologist and /or by an Autism Diagnostic Interview-Revised (ADI-R) or Autism Diagnostic Observation Schedule (ADOS) .
Overnight urine with measurement of quantity and time will be collected and a little part of the urinary samples will be frozen for storage till analysis. Age, weight and length of the children at collection time will be ascertained.
Four stratified groups (autistic boys, girls, and healthy controls, boys and girls, each age 5-15) will be formed.
Urinary samples will further remain anonymous through an Identification-Number (ID).
Analysis of hormones and the most of the known metabolites excreted in urine will be performed by a specialised laboratory using gas chromatography-mass spectrometry (GC/MS) analysis (University Hospital Bern, Steroid Laboratory, Switzerland).
The statistical analysis, with age, gender, weight, diagnosis and hormone analysis results as factors , consists in linear regression analysis to detect deviation in hormone formation and metabolism.
|Intersci Research Association|
|Leibnitz, Steiermark, Austria, 8430|
|Principal Investigator:||Johann Kurz, MD||Intersci Research Association, Austria|