CXCL9, CXCL10, CXCL11, and CXCR3 in Dry Eye Syndrome - Full Text View

Sponsor:	Chonnam National University Hospital
Information provided by:	Chonnam National University Hospital
ClinicalTrials.gov Identifier:	NCT00991679

Purpose

The aim of this study is to investigate the expression of CXCL9, CXCL10, CXCL11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome.

Thirty-three patients with dry eye (16 Sjögren's syndrome and 17 non-Sjögren's syndrome patients) and 15 control subjects were recruited. The concentrations of CXCL9, CXCL10, and CXCL11 in tears were measured using enzyme-linked immunosorbent assay. The correlation between chemokine levels and tear film and ocular surface parameters was analyzed. Expression of CXCL9, CXCL10, CXCL11, and CXCR3 in the conjunctiva was evaluated using immunohistochemistry. Flow cytometry was performed to count CXCR3+ cells and CXCR3+CD4+ cells in the conjunctiva.

Condition
Dry Eye Syndrome

Study Type:	Observational
Study Design:	Observational Model: Case Control Time Perspective: Prospective
Official Title:	Expression of CXCL9, CXCL10, CXCL11, and CXCR3 in the Tear Film and Ocular Surface of Patients With Dry Eye Syndrome

Further study details as provided by Chonnam National University Hospital:

Biospecimen Retention: Samples Without DNA

Biospecimen Description:

tears, conjunctiva

Enrollment:	48
Study Start Date:	February 2008
Study Completion Date:	December 2008
Primary Completion Date:	October 2008 (Final data collection date for primary outcome measure)

Groups/Cohorts
Normal control subjects Normal control subjects who did not show the clinical symptom and findings of dry eye syndrome.
dry eye patients Patients with dry eye syndrome who had symptoms of dry eye for more than 3 months, low tear film break up time (BUT, ≤7 sec), low Schirmer test (<10 mm), low tear clearance rate (<8X), and positive fluorescein or rose bengal vital staining (≥3) and were not treated with anti-inflammatory agents such as topical cyclosporine or steroids were included in the study.

Eligibility

Ages Eligible for Study:	24 Years to 77 Years
Genders Eligible for Study:	Both
Accepts Healthy Volunteers:	Yes
Sampling Method:	Non-Probability Sample

Study Population

Primary care clinic

Criteria

Inclusion Criteria:

clinical diagnosis of dry eye syndrome

Exclusion Criteria:

active ocular infection or inflammation not associated with dry eye
drug toxicity
contact lens wear
ocular allergy
ocular surgery within the last 3 months
lid or lash abnormalities

Contacts and Locations

Please refer to this study by its ClinicalTrials.gov identifier: NCT00991679

Locations

Korea, Republic of, Chonnam
Chonnam National University Hospital
Gwangju, Chonnam, Korea, Republic of, 501-757

Sponsors and Collaborators

Chonnam National University Hospital

Investigators

Study Director:

Kyung-Chul Yoon, MD, PhD

Department of Ophthalmology, Chonnam National University Medical School and Hospital, Gwangju, South Korea

More Information

Publications:

Yoon KC, De Paiva CS, Qi H, Chen Z, Farley WJ, Li DQ, Pflugfelder SC. Expression of Th-1 chemokines and chemokine receptors on the ocular surface of C57BL/6 mice: effects of desiccating stress. Invest Ophthalmol Vis Sci. 2007 Jun;48(6):2561-9.

Wallace GR, John Curnow S, Wloka K, Salmon M, Murray PI. The role of chemokines and their receptors in ocular disease. Prog Retin Eye Res. 2004 Jul;23(4):435-48. Review.

Pflugfelder SC, Jones D, Ji Z, Afonso A, Monroy D. Altered cytokine balance in the tear fluid and conjunctiva of patients with Sjögren's syndrome keratoconjunctivitis sicca. Curr Eye Res. 1999 Sep;19(3):201-11.

Additional publications automatically indexed to this study by National Clinical Trials Identifier (NCT ID):

Yoon KC, Park CS, You IC, Choi HJ, Lee KH, Im SK, Park HY, Pflugfelder SC. Expression of CXCL9, -10, -11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome. Invest Ophthalmol Vis Sci. 2010 Feb;51(2):643-50. Epub 2009 Oct 22.

Responsible Party:	Department of Ophthalmology, Chonnam National University Medical School and Hospital, Gwangju, South Korea ( Kyung-Chul Yoon, MD/ Assisted professor )
Study ID Numbers:	I-2007-12-111
Study First Received:	October 7, 2009
Last Updated:	October 7, 2009
ClinicalTrials.gov Identifier:	NCT00991679 History of Changes
Health Authority:	Korea: Institutional Review Board

Keywords provided by Chonnam National University Hospital:

Dry eye syndrome
CXCL9 chemokine
CXCL10 chemokine
CXCL11 chemokine
CXCR3 receptor

Additional relevant MeSH terms:

Eye Diseases
Lacrimal Apparatus Diseases
Dry Eye Syndromes

ClinicalTrials.gov processed this record on March 18, 2010