Abundance and Distribution of Lipids and Proteins in Nonalcoholic Fatty Liver Disease (NAFLD)
Nonalcoholic fatty liver disease (NAFLD) is the most common type of liver disease in the United States. The incidence of NAFLD is very similar to that of obesity, type 2 diabetes, and the metabolic syndrome. The investigators hypothesize that there may be a relationship between over-nutrition, decreased physical activity and the development of fatty liver. The purpose of this study is to identify the types of fats and proteins, and the quantity of each, that are associated with increased severity of NAFLD.
Nonalcoholic Fatty Liver Disease
|Study Design:||Observational Model: Case Control
Time Perspective: Prospective
|Official Title:||Relative Abundance and Spatial Distribution of Lipids and Proteins in Nonalcoholic Fatty Liver Disease (NAFLD)|
- To identify and relatively quantify, using high performance liquid chromatography electrospray ionization mass spectrometry, select lipids and proteins present in human liver biopsies. [ Time Frame: 2 years ] [ Designated as safety issue: No ]
- To spatially profile, using matrix assisted laser desorption ionization time of flight mass spectrometry, select lipids and proteins present in human liver biopsies. [ Time Frame: 2 years ] [ Designated as safety issue: No ]
Biospecimen Retention: Samples With DNA
liver biopsy, blood, plasma
|Study Start Date:||October 2009|
|Estimated Study Completion Date:||November 2014|
|Estimated Primary Completion Date:||November 2014 (Final data collection date for primary outcome measure)|
Obese, bariatric surgery, liver biopsy
Obese subjects approved and scheduled for bariatric surgery at Vanderbilt University Medical Center
Normal BMI, abdominal surgery, liver biopsy
Normal weight subjects having elective abdominal surgery at Vanderbilt University Medical Center.
Liver transplantation donors and recipients
All livers made available for implantation or explantation will be eligible.
|United States, Tennessee|
|Vanderbilt University Medical Center|
|Nashville, Tennessee, United States, 37232|
|Principal Investigator:||Charles R Flynn, PhD||Vanderbilt University|
|Study Chair:||Najji Abumrad, MD||Vanderbilt University|