Metabolome and Microbiomic in Fecal Samples (MicroMet)
The purpose of this study is to evaluate the effects of storage and handling on fecal samples collected for metabolomic, proteomic and microbiomic and analysis.
|Study Design:||Time Perspective: Prospective|
|Official Title:||Fecal Samples for Metabolomic and Microbiomic Studies: Effects of Preparation and Storage|
- Microbiome and metabolome yield [ Time Frame: one month ] [ Designated as safety issue: No ]
Biospecimen Retention: None Retained
|Study Start Date:||April 2009|
|Study Completion Date:||May 2009|
|Primary Completion Date:||May 2009 (Final data collection date for primary outcome measure)|
Microbiome, Metabolome, Stool, Toddler
healthy infant stool samples
Human fecal collection for clinical or research studies usually occurs at the residence of the subject, an environment not equipped with measures to accommodate proper preparation and storage of samples for microbiome and metabolome analysis.
To evaluate the effects of storage and handling on fecal samples collected for metabolomic, proteomic and microbiomic and analysis.
Fecal samples will be collected within several minutes of voiding from healthy infants at the time of diaper change at day-care center.
Samples will be divided into 7 tubes, and subjected to different storage temperatures, before preforming microbial analysis by DNA sequencing, and metabolome analysis by H1NMR spectroscoty.
|United States, Massachusetts|
|MassGeneral for Children|
|Boston, Massachusetts, United States, 02114|
|Principal Investigator:||Harland Winter, MD||MGH|