Granzymes and Perforin at the Onset of Chronic Obstructive Pulmonary Disease (COPD) Exacerbations
COPD exacerbations are characterized by an excessive accumulation and activation of inflammatory cells in the airways. It is not known whether this phenomenon represents a risk for for lung damage via the release in the extracellular environment of potent cytolitic cellular granular contents such as granzymes and perforin.
The investigators assess the intracellular expression of granzymes and perforin in neutrophils and large granular lymphocytes (LGL) at the onset of exacerbations compared to stable disease.
The investigators hypothesize that a greater release of intracellular perforin and granzymes from neutrophils and LGL into the extracellular environment occur at exacerbations compared to stable condition and that these changes are more pronounced in COPD patients than in subjects without COPD who undergo respiratory infection.
Chronic Obstructive Pulmonary Disease
|Study Design:||Observational Model: Case Control
Time Perspective: Prospective
|Official Title:||Granzymes and Perforin at the Onset of COPD Exacerbations|
- Expression of granzymes and perforin in neutrophiles and LGL sputum cells [ Time Frame: 6 months ] [ Designated as safety issue: No ]
Biospecimen Retention: Samples Without DNA
sputum cells and supernatant
|Study Start Date:||December 2002|
|Study Completion Date:||September 2009|
|Primary Completion Date:||August 2008 (Final data collection date for primary outcome measure)|
COPD patients who undergo exacerbation
Non COPD patients (controls)
Subjects who undergo respiratory infection (acute bronchitis) without COPD or other respiratory illness
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