Longitudinal Study of the Human Intestinal Microbiome - Full Text View

Sponsor:	National Institute of Allergy and Infectious Diseases (NIAID)
Information provided by:	National Institute of Allergy and Infectious Diseases (NIAID)
ClinicalTrials.gov Identifier:	NCT00832286

Purpose

The purpose of this study is to identify the human intestinal microbiota (microbes that live inside and on human bodies) in healthy adults over a 6-month period and to study the effect of an antibiotic on the intestinal microbiota. Participants will include up to 60 healthy adult subjects, ages 18-45 years, from the Baltimore and University of Maryland communities. Study procedures will include providing multiple stool samples throughout the study. Participants will take a licensed antibiotic, Ciprofloxacin, for 3 days. Participants may be involved in study related procedures for up to 7 months.

Condition	Intervention
Intestinal Microbiome	Drug: Ciprofloxacin hydrochloride

Study Type:	Interventional
Study Design:	Allocation: Non-Randomized Intervention Model: Single Group Assignment Masking: Open Label
Official Title:	Longitudinal Study of the Human Intestinal Microbiome Before and After Antibiotic Administration

Resource links provided by NLM:

Genetics Home Reference related topics: Help Me Understand Genetics

MedlinePlus related topics: Antibiotics

Drug Information available for: Ciprofloxacin Ciprofloxacin hydrochloride

U.S. FDA Resources

Further study details as provided by National Institute of Allergy and Infectious Diseases (NIAID):

Primary Outcome Measures:

Analysis of GI microbial community composition using the Affymetrix PhyloChip Platform. [ Time Frame: Analysis. ] [ Designated as safety issue: No ]
Isolation of Genomic DNA from stool samples. [ Time Frame: Weekly stool samples will be obtained for over a 6-month period at the following intervals: Day 0, Weeks 1, 2, 3, 4, 8, 12, 13, 14, 15, 16, 20, and 24. ] [ Designated as safety issue: No ]
Surveys of GI microbiome diversity using 16S rDNA analysis at the Institute of Genome Sciences. [ Time Frame: Analysis. ] [ Designated as safety issue: No ]

Enrollment:	60
Study Start Date:	July 2009
Study Completion Date:	January 2011
Primary Completion Date:	September 2010 (Final data collection date for primary outcome measure)

Arms	Assigned Interventions
Experimental: Cipro At Week 12, subjects will receive a 3-day course of oral Ciprofloxacin 500 mg every 12h.	Drug: Ciprofloxacin hydrochloride Licensed medication, dose: 500 mg every 12 hours for 3 days.

Detailed Description:

The purpose of this study is to describe the intestinal microbiomes of a cohort of healthy adult subjects over a 6-month period of time. Many questions about the human microbiota exist. Previous studies have shown that the differences among individuals are greater than the differences among different sampling sites in a single individual. Up to 60 healthy adult subjects, ages 18-45 years, from the Baltimore and University of Maryland Baltimore communities will be recruited and screened to document their health status. A brief medical history, including recent travel and antibiotic use, will be recorded. Subjects will provide a stool specimen for genomic analysis of the intestinal microbiome over a 6-month period at the following intervals: Day 0, Week 1, Week 2, Week 3, Week 4, Week 8, Week 12, Week 13, Week 14, Week 15, Week 16, Week 20, and Week 24. At Week 12, subjects will receive a 3-day course of oral Ciprofloxacin 500 mg every12 hours. Weekly stool specimens will be obtained beginning with the first day of antibiotic use and for 4 Weeks thereafter. Then monthly specimens of stool and other sites will be resumed at Week 16. The primary objective is to define the human intestinal microbiome in healthy adults in a longitudinal fashion. The secondary objective is to define the re-colonization of the intestine after treatment with a broad-spectrum antibiotic using comprehensive genomic techniques. Each subject will participate in the study for up to 7 months.

Eligibility

Ages Eligible for Study:	18 Years to 45 Years
Genders Eligible for Study:	Both
Accepts Healthy Volunteers:	Yes

Criteria

Inclusion Criteria:

Male or female, ages 18 to 45 years, inclusive.
Healthy as determined by screening medical history, medication history, and absence of acute illness such as gastrointestinal or respiratory infection.
Capable of understanding, consenting and complying with the entire study protocol.
Provide voluntary written Informed Consent.
Females of childbearing potential are required to utilize an appropriate method of contraception [abstinence, oral contraceptives, IUD, condoms with spermicidal foam, surgical sterilization depots and injectable contraceptives, or diaphragms with spermicidal jelly or cream] 30 days prior to the Week 12 visit.

Exclusion Criteria:

Chronic diarrhea, inflammatory bowel disease, irritable bowel syndrome, or other gastrointestinal disorder, gastrointestinal surgery (except appendectomy, polypectomy, or herniorraphy), or severe chronic illness such as major organ failure, diabetes, HIV/AIDS.
Female who is pregnant or lactating; or a female subject with a positive urine pregnancy test determined at the Week 12 visit.
History of hypersensitivity to Ciprofloxacin, any member of the quinolone class of antimicrobial agents, or any compound of the product.
History of tendinitis or tendon rupture.
History of seizures other than febrile seizure as a young child.
Treatment with antibiotics within one month before the initial specimen collection.
History of clinically significant acute or chronic illness or other condition requiring chronic medication therapy (including systemic but not intranasal steroids), except for birth control pills, inhalers, anti-anxiety or anti-depression medications.
History of cardiac rhythm abnormalities or QT prolongation or a family history of cardiac rhythm abnormalities or sudden unexplained death.
History of current or past use of theophylline for asthma or tizanidine, due to known interaction with Ciprofloxacin.
History of spasticity (due to the potential for requiring tizanidine treatment), asthma, chronic bronchitis, emphysema, and other lung diseases (due to potential for requiring theophylline [or dimethylxanthine] treatment).
Medical, occupational, or family problems as a result of alcohol or illicit drug use during the past 12 months.

Contacts and Locations

Please refer to this study by its ClinicalTrials.gov identifier: NCT00832286

Locations

United States, Maryland
University of Maryland School of Medicine
Baltimore, Maryland, United States, 21201

Sponsors and Collaborators

National Institute of Allergy and Infectious Diseases (NIAID)

More Information

No publications provided

Responsible Party:	Robert Johnson, HHS/NIAID/DMID
ClinicalTrials.gov Identifier:	NCT00832286 History of Changes
Other Study ID Numbers:	07-0053, Microbiome CVD 1000, N01AI40014C
Study First Received:	January 29, 2009
Last Updated:	August 11, 2011
Health Authority:	United States: Federal Government United States: Food and Drug Administration United States: Institutional Review Board

Keywords provided by National Institute of Allergy and Infectious Diseases (NIAID):

intestinal microbiome, genomic techniques

Additional relevant MeSH terms:

Ciprofloxacin
Nucleic Acid Synthesis Inhibitors
Enzyme Inhibitors
Molecular Mechanisms of Pharmacological Action

Pharmacologic Actions
Anti-Infective Agents
Therapeutic Uses

ClinicalTrials.gov processed this record on May 23, 2012