MOR and COMT SNP Polymorphism and Pain
Recruitment status was Recruiting
Patients with certain polymorphism in the MOR and COMT genes will display differences in their response to analgesics.
|Study Design:||Observational Model: Cohort
Time Perspective: Prospective
|Official Title:||Does mu Opioid Receptor (MOR) and Catechol-O-methyltransferase (COMT) Genes Polymorphism Correlate of Clinical Postoperative Pain and Response to Analgesics|
- Postoperative assessments include PCA use (e.g., number of patient demands, total morphine administered) in each 24-h interval during the 48-h study period - primary endpoint. [ Time Frame: 48 hours ] [ Designated as safety issue: No ]
- No secondary outcome endpoint [ Time Frame: no time frame ] [ Designated as safety issue: No ]
Biospecimen Retention: Samples With DNA
|Study Start Date:||October 2008|
|Estimated Study Completion Date:||December 2009|
|Estimated Primary Completion Date:||October 2009 (Final data collection date for primary outcome measure)|
Other: SNP genotyping
Coded blood specimens will be transported to the Department of Gene Technology TTÜ and genotyping analysis will be performed. Lymphocytes will be isolated from blood specimens using Ficol-Paq gradients, and genomic DNA isolated using a salting-out procedure. Variants of the MOR gene and other genes of interests will be performed by DNA sequence analysis of PCR-amplified DNA, using primers located in flanking intron sequence. All methods proposed are currently in operation in the respective facilities.
Other Name: SNP genotyping
After tissue injury, there is great interindividual variability among patients in the amount of pain experienced (pain intensity and duration of pain) and in the degree of pain relief from analgesics. In experimental settings, Single Nucleotide Polymorphisms (SNP) at the MOR and COMT genes have been found to alter the response to opioids in in vitro models and in human.We will collect clinical data on one hundred patients undergoing surgery. We will obtain DNA extracted via PCR techniques from the patients' blood and we will identify SNPs at the mu opioid receptor and catechol-O-methyltransferase genes. We will analyze the data to search for correlation between clinical patterns of postoperative pain and opioid effects and SNPs.
|Yuri Kolesnikov MD PhD||Recruiting|
|Tallinn, Estonia, 11312|
|Contact: Peeter Ross, MD 372 6973002 firstname.lastname@example.org|
|Contact: Yuri Kolesnikov 372 6973002 email@example.com|
|Sub-Investigator: Boris Gabovits, MD|
|Principal Investigator: Yuri Kolesnikov, MD PhD|
|Study Director:||Yuri Kolesnikov, MD PhD||ETCH|