The Evaluation of Thiol Redox Status in Conjunctiva of Dry Eye Patients
Recruitment status was Not yet recruiting
Dry eye disease is related with oxidative stress at the ocular surface. GSH is one of the key factor of protect from oxidative stress. To evaluate thiol status of ocular surface cells by monitoring GSH concentration, we can evaluate the severity of dry eye.
Dry Eye Disease
|Study Design:||Observational Model: Case Control|
|Official Title:||The Evaluation of Thiol Redox Status in Conjunctiva of Dry Eye Patients|
- Tear amount, tear break up time, and so on are measured clinically. The thiol redox status is measured by staining the membrane. [ Time Frame: Once a month (totally 2-4 times) at the clinic ] [ Designated as safety issue: Yes ]
Biospecimen Retention: Samples Without DNA
After application of anethesia eyedrops, nitrocel membrane is put ocular surface. Then, removed the membrane. This method is commonly used clinically.
|Study Start Date:||August 2008|
|Estimated Study Completion Date:||March 2010|
|Estimated Primary Completion Date:||December 2008 (Final data collection date for primary outcome measure)|
Sjogren syndrome with dry eye
dry eye without Sjogren syndrome
Dry eye by shortened tear break up time
The conjunctival cells are collected by impression cytology. Those cells are stained with 100 mM of monochlorobimane (MCB, M-1381, Molecular Probes, Eugene, OR) and propidium iodide (PI). Fluorescent intensity, reflecting the amount of icGSH, was inspected under a confocal laser scan microscope. To detect the GSH levels we used a fluorescent MCB probe with excitation and emission wavelengths of 405 nm and 410-480 nm, respectively. The cell-permeating MCB probe is non-fluorescent but forms a fluorescent adduct with GSH in a reaction catalyzed by glutathione-S-transferase.
|Contact: Jun Yamada, MD, PhD.||+email@example.com|
|Contact: Norihiko Yokoi, MD, PhD.||+firstname.lastname@example.org|
|Kyoto Prefectural University of Medicine hospital||Recruiting|
|Contact: Shigeru Kinoshita, MD, PhD. +81-75-251-5577 email@example.com|