Cellular Proteome From Leukocytes of Glaucoma Patients in Comparison With Patients With Parkinson's Disease

This study has been withdrawn prior to enrollment.
(was replaced by an other study)
Sponsor:
Collaborator:
Friedrich-Wilhelms-University of Bonn, Germany
Information provided by:
University Hospital, Basel, Switzerland
ClinicalTrials.gov Identifier:
NCT00327301
First received: May 17, 2006
Last updated: June 23, 2009
Last verified: June 2009
  Purpose

Glaucoma is a worldwide leading cause of blindness. The key feature of this ocular neuropathy is characterized by an excavating optic nerve head. Loss of retinal ganglion cells is the final end point in blinding diseases of the optic nerve such as glaucoma. It is known that neuronal cell death in glaucoma occurs by an apoptotic mechanism. In earlier studies the investigators could demonstrate that the process of apoptosis is reflected in circulating leukocytes by different parameters, like differential mRNA expression and an increased fragmentation of the DNA. Such alterations point out a relationship between cellular stress and apoptotic events.

Based on the results of mRNA-expression the investigators also expect alterations on the protein level.

This study is, therefore, designed to characterize the proteome related to the proteins involved in cell death related pathways.

Thus, the expression pattern of several proteins in leukocytes from patients with primary open angle glaucoma will be analyzed by techniques like Western-blot and tandem mass spectrometry. These samples will be compared with samples from healthy controls. In addition, they will also be compared with samples from patients with Parkinson's disease. Since glaucoma is a neurodegenerative disease, these patients will be included as positive controls in this study.


Condition
Parkinson's Disease
Glaucoma

Study Type: Observational
Study Design: Observational Model: Case-Only
Time Perspective: Prospective
Official Title: Systematic Characterization of the Cellular Proteome From Human Leukocytes of Glaucoma Patients in Comparison With Patients With Parkinson's Disease

Resource links provided by NLM:


Further study details as provided by University Hospital, Basel, Switzerland:

Biospecimen Retention:   None Retained

plasma


Estimated Enrollment: 80
Study Start Date: October 2006
Primary Completion Date: May 2008 (Final data collection date for primary outcome measure)
Detailed Description:

Hypothesis:

Differences in the proteome concerning cell death pathways of glaucoma patients correspond to the differences in the mRNA expression of these patients.

Specific aims:

Characterization of the cellular proteome from human leukocytes of glaucoma patients compared to healthy controls and patients with Parkinson's disease.

Background:

Glaucoma is a worldwide leading cause of blindness. The key feature of this ocular neuropathy is characterized by an excavating optic nerve head. Loss of retinal ganglion cells is the final end point in blinding diseases of the optic nerve such as glaucoma. It is known that neuronal cell death in glaucoma occurs by an apoptotic mechanism. In earlier studies we could demonstrate that this cell death is reflected in circulating leukocytes by different parameters, like differential mRNA expression, and an increased fragmentation of the DNA. The differences in mRNA expression indicate a close relationship to cellular stress conditions and apoptotic events: increased mRNA expression was detected for p53, 20S proteasome alpha subunit, ABC1 transporter, p21(WAF1/CIP1), 14-3-3 sigma factor, MMP-9 and MMP-14, and TIMP-1.

Based on the assumption that glaucoma patients may differ on the level of their expression for these mRNAs, we expect that similar differences should exist at the protein level.

This study is, therefore, designed to characterize the proteome related to the proteins involved in cell death related pathways.

  Eligibility

Ages Eligible for Study:   18 Years to 85 Years
Genders Eligible for Study:   Both
Accepts Healthy Volunteers:   Yes
Sampling Method:   Non-Probability Sample
Study Population

glaucoma patients

Criteria

Inclusion Criteria:

  • German native speakers
  • Age between 18-85 years
  • Primary open-angle glaucoma (POAG) with and without vasospasm
  • An inclusion criterion for one control group is Parkinson's disease.

Exclusion Criteria:

Any history of:

  • Ocular or systemic diseases other than glaucoma or Parkinson's disease
  • Drug or alcohol abuse
  • Any condition potentially interfering with the visual field results. Visual fields will be obtained from the chart.
  • Mental impairment interfering with the ability to cooperate and understand the purpose of this study; exception: those patients with Parkinson's disease. A prerequisite for including patients with Parkinson's disease in this study is the ability of these patients to cooperate and completely understand the purpose of this study.
  Contacts and Locations
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the Contacts provided below. For general information, see Learn About Clinical Studies.

Please refer to this study by its ClinicalTrials.gov identifier: NCT00327301

Sponsors and Collaborators
University Hospital, Basel, Switzerland
Friedrich-Wilhelms-University of Bonn, Germany
Investigators
Study Director: Selim Orgül, MD University Hospital, Basel, Switzerland
  More Information

No publications provided

Responsible Party: Selim Orgul, University Hospital, Basel, Switzerland
ClinicalTrials.gov Identifier: NCT00327301     History of Changes
Other Study ID Numbers: 075-WUK-2006-001
Study First Received: May 17, 2006
Last Updated: June 23, 2009
Health Authority: Switzerland: Swissmedic

Keywords provided by University Hospital, Basel, Switzerland:
POAG
Parkinson's disease
cellular proteome
human leucocytes
vasospasm
POAG patients
Patients with Parkinson's disease
Healthy subjects

Additional relevant MeSH terms:
Glaucoma
Parkinson Disease
Ocular Hypertension
Eye Diseases
Parkinsonian Disorders
Basal Ganglia Diseases
Brain Diseases
Central Nervous System Diseases
Nervous System Diseases
Movement Disorders
Neurodegenerative Diseases

ClinicalTrials.gov processed this record on July 20, 2014