Rickettsial Genesis to Sarcoidosis in Denmark
One study has suggested a connection between an infection with Rickettsia species and sarcoidosis finding molecular evidence of rickettsial deoxyribonucleic acid (DNA) in two patients. Another study found no antibodies in twenty Swedish patients with sarcoidosis.
This study will further enhance the subject by three different approaches in Danish patients.
The purpose of this study is to find serological and molecular evidence of an infection with Rickettsia species in Danish patients with sarcoidosis.
|Study Design:||Observational Model: Case Control
Time Perspective: Prospective
|Official Title:||Rickettsial Genesis to Sarcoidosis in Denmark|
Buffy coats, serum, bronchoalveolar lavage fluid and transbronchial biopsies
|Study Start Date:||September 2006|
|Study Completion Date:||March 2009|
|Primary Completion Date:||January 2009 (Final data collection date for primary outcome measure)|
Patients without sarcoidosis, but requiring a fiberoptic bronchoscopy
Patients with newly diagnosed sarcoidosis
The study has three approaches to the subject:
- A serological survey of rickettsial immunoglobin G (IgG)-antibodies in 50 patients with sarcoidosis compared to 50 blood donors without sarcoidosis.
- An archival study using pre-existing lymph node biopsies, where immunohistochemical analysis, fluorescent in situ hybridization, and polymerase chain reaction will be applied to samples from 50 patients with sarcoidosis and compared to 50 patients without sarcoidosis and 10 patients with tuberculosis.
- A prospective study using bronchoscopically obtained samples from 20 patients with sarcoidosis, compared to 20 patients without sarcoidosis. The study applies the same techniques as the second study, but enhancing the information from the patients.
Please refer to this study by its ClinicalTrials.gov identifier: NCT00326534
|Copenhagen S, Denmark, 2300|
|Principal Investigator:||Claus B Svendsen, MD||Statens Serum Institut|
|Study Chair:||Nils Milman, MD||Rigshospitalet, Denmark|
|Study Chair:||Karen A Krogfelt, PhD||Statens Serum Institut|